Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3945971..3946329 Replicon chromosome
Accession NZ_CP012633
Organism Escherichia coli strain SF-166

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag AN204_RS28235 Protein ID WP_001332330.1
Coordinates 3945971..3946093 (-) Length 41 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AN204_RS19335 Protein ID WP_000125566.1
Coordinates 3946096..3946329 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN204_RS19305 3941453..3942739 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
AN204_RS19310 3942739..3943728 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AN204_RS19315 3943725..3944546 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AN204_RS19320 3944549..3944926 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AN204_RS19325 3944933..3945781 + 849 WP_000257201.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AN204_RS28230 3945778..3945930 - 153 WP_146692624.1 CcdB family protein -
AN204_RS28235 3945971..3946093 - 123 WP_001332330.1 CcdB family protein Toxin
AN204_RS19335 3946096..3946329 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AN204_RS19340 3946415..3946894 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
AN204_RS19345 3947086..3948948 - 1863 WP_000377122.1 glutathione-regulated potassium-efflux system protein KefC -
AN204_RS19350 3948941..3949471 - 531 WP_000600749.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AN204_RS19355 3949579..3950910 - 1332 WP_001183207.1 MFS transporter -
AN204_RS19360 3950969..3951256 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-39)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 41 a.a.        Molecular weight: 4563.34 Da        Isoelectric Point: 10.9266

>T56865 WP_001332330.1 NZ_CP012633:c3946093-3945971 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Download         Length: 123 bp

>T56865 NZ_CP012633:c3946093-3945971 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTGTTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT56865 WP_000125566.1 NZ_CP012633:c3946329-3946096 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT56865 NZ_CP012633:c3946329-3946096 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References