Detailed information of TA system TA056832

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3017388..3018186
• Replicon: chromosome
• Accession: NZ_CP012631
• Organism: Escherichia coli strain SF-173

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1RDD2
• Locus tag: AN205_RS15195
• Protein ID: WP_001094434.1
• Coordinates: 3017388..3017762 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AN205_RS15200
• Protein ID: WP_001552747.1
• Coordinates: 3017809..3018186 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN205_RS15160	3012977..3013531	-	555	WP_001001909.1	molecular chaperone YcdY	-
AN205_RS15165	3013555..3014292	-	738	WP_000283664.1	zinc-binding phosphatase	-
AN205_RS15170	3014347..3015285	-	939	WP_000351298.1	glyoxylate/hydroxypyruvate reductase GhrA	-
AN205_RS15180	3015756..3016598	-	843	WP_001280460.1	DUF4942 domain-containing protein	-
AN205_RS15185	3016691..3016888	-	198	WP_000839257.1	DUF957 domain-containing protein	-
AN205_RS15190	3016900..3017391	-	492	WP_000976859.1	hypothetical protein	-
AN205_RS15195	3017388..3017762	-	375	WP_001094434.1	TA system toxin CbtA family protein	Toxin
AN205_RS15200	3017809..3018186	-	378	WP_001552747.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
AN205_RS15205	3018236..3018880	-	645	WP_000086759.1	hypothetical protein	-
AN205_RS15210	3018899..3019120	-	222	WP_000692315.1	DUF987 domain-containing protein	-
AN205_RS15215	3019183..3019659	-	477	WP_001186727.1	RadC family protein	-
AN205_RS15220	3019675..3020148	-	474	WP_001542276.1	antirestriction protein	-
AN205_RS15225	3020242..3020487	-	246	WP_001164966.1	hypothetical protein	-
AN205_RS15230	3020487..3021308	-	822	WP_001234585.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13921.82 Da
• Isoelectric Point: 6.8517

>T56832 WP_001094434.1 NZ_CP012631:c3017762-3017388 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T56832 NZ_CP012631:c3017762-3017388 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13757.63 Da
• Isoelectric Point: 6.3127

>AT56832 WP_001552747.1 NZ_CP012631:c3018186-3017809 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRTGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT56832 NZ_CP012631:c3018186-3017809 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCACCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCTGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCACCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: Q1RDD2