Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3708281..3708899 Replicon chromosome
Accession NZ_CP012625
Organism Escherichia coli strain SF-468

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AN206_RS18950 Protein ID WP_001291435.1
Coordinates 3708681..3708899 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AN206_RS18945 Protein ID WP_000344800.1
Coordinates 3708281..3708655 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN206_RS18935 3703371..3704564 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AN206_RS18940 3704587..3707736 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AN206_RS18945 3708281..3708655 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AN206_RS18950 3708681..3708899 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AN206_RS18955 3709073..3709624 + 552 WP_000102543.1 maltose O-acetyltransferase -
AN206_RS18960 3709740..3710210 + 471 WP_000136192.1 YlaC family protein -
AN206_RS18965 3710374..3711924 + 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AN206_RS18970 3711966..3712319 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AN206_RS18980 3712698..3713009 + 312 WP_000409908.1 MGMT family protein -
AN206_RS18985 3713040..3713612 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T56805 WP_001291435.1 NZ_CP012625:3708681-3708899 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T56805 NZ_CP012625:3708681-3708899 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT56805 WP_000344800.1 NZ_CP012625:3708281-3708655 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT56805 NZ_CP012625:3708281-3708655 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References