Detailed information of TA system TA056782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/COG3657-dnstrm_HI1420
• Location: 1675196..1675800
• Replicon: chromosome
• Accession: NZ_CP012606
• Organism: Ralstonia insidiosa strain FC1138

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S9TBH7
• Locus tag: ACS15_RS26065
• Protein ID: WP_021193105.1
• Coordinates: 1675501..1675800 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ACS15_RS26060
• Protein ID: WP_021193106.1
• Coordinates: 1675196..1675498 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACS15_RS26035	1670749..1672368	+	1620	WP_021193111.1	AMP-binding protein	-
ACS15_RS26040	1672365..1672781	+	417	WP_021193110.1	acyl-CoA thioesterase	-
ACS15_RS26045	1672860..1673198	+	339	WP_064496325.1	RidA family protein	-
ACS15_RS26050	1673248..1673538	-	291	WP_037010984.1	hypothetical protein	-
ACS15_RS26055	1673693..1674997	-	1305	WP_021193107.1	metabolite/H+ symporter	-
ACS15_RS26060	1675196..1675498	-	303	WP_021193106.1	putative addiction module antidote protein	Antitoxin
ACS15_RS26065	1675501..1675800	-	300	WP_021193105.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACS15_RS26070	1675899..1677128	-	1230	WP_021193104.1	MFS transporter	-
ACS15_RS26075	1677267..1678100	-	834	WP_021193103.1	GntR family transcriptional regulator	-
ACS15_RS26080	1678291..1678833	-	543	WP_031328796.1	helix-turn-helix transcriptional regulator	-
ACS15_RS26085	1678966..1679544	+	579	WP_021193101.1	GNAT family N-acetyltransferase	-
ACS15_RS26090	1679792..1680235	+	444	WP_021193100.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-85)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-95)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11245.93 Da
• Isoelectric Point: 9.7981

>T56782 WP_021193105.1 NZ_CP012606:c1675800-1675501 [Ralstonia insidiosa]
MLTIRTTEVFDAWFAKLRDKTAQRRIQVRIDRLQLGNPGDAKAVRDGIGELRIDHGPGYRVYFVQRGAVLIILLCGGDKS
TQEADIRRAIDLSRHLDVE

Nucleotide

• Length: 300 bp

>T56782 NZ_CP012606:c1675800-1675501 [Ralstonia insidiosa]
ATGCTGACGATCCGCACCACCGAAGTGTTCGATGCCTGGTTCGCGAAGCTGCGTGACAAGACTGCGCAGCGGCGCATCCA
GGTGCGCATCGACCGTCTGCAACTGGGCAATCCGGGCGATGCGAAGGCGGTGCGCGATGGCATCGGTGAGCTTCGGATCG
ATCATGGCCCCGGCTATCGCGTGTATTTCGTACAGCGTGGCGCGGTGCTGATCATCCTGCTTTGCGGCGGCGACAAGTCG
ACACAGGAAGCGGACATCCGGCGCGCGATCGACTTGTCGCGCCATCTCGACGTGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10887.45 Da
• Isoelectric Point: 5.6269

>AT56782 WP_021193106.1 NZ_CP012606:c1675498-1675196 [Ralstonia insidiosa]
MPTDKSVKTRLWDSAEHLKTEADMAEYLDACLEEAGDDPAFITHALGVVARARGMTQLARDTGMTREGLYKALSEEGNPS
FATVLKVMRALGIRLHAVPA

Nucleotide

• Length: 303 bp

>AT56782 NZ_CP012606:c1675498-1675196 [Ralstonia insidiosa]
ATGCCGACCGACAAATCTGTCAAAACCCGCCTCTGGGACTCTGCCGAACACCTCAAAACCGAGGCCGACATGGCCGAGTA
TCTCGACGCCTGCCTCGAAGAAGCCGGCGATGACCCCGCCTTCATCACGCACGCGCTGGGCGTGGTTGCTCGTGCACGCG
GCATGACGCAACTCGCGCGTGACACTGGCATGACACGTGAAGGCCTCTACAAAGCACTGTCGGAAGAAGGCAATCCAAGC
TTCGCCACCGTGCTCAAGGTCATGCGTGCGTTGGGTATTCGGTTACACGCTGTGCCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A117DRT1