Detailed information of TA system TA056781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1481750..1482341
• Replicon: chromosome
• Accession: NZ_CP012606
• Organism: Ralstonia insidiosa strain FC1138

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: S9S4M8
• Locus tag: ACS15_RS25200
• Protein ID: WP_021193282.1
• Coordinates: 1481750..1482046 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ACS15_RS25205
• Protein ID: WP_021193281.1
• Coordinates: 1482048..1482341 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACS15_RS25175	1477108..1477728	+	621	WP_031328841.1	cytochrome c oxidase subunit 3	-
ACS15_RS25180	1477725..1478417	+	693	WP_021193286.1	heme-copper oxidase subunit III family protein	-
ACS15_RS25185	1478426..1478776	+	351	WP_021193285.1	cytochrome C oxidase subunit IV family protein	-
ACS15_RS25190	1478927..1480240	+	1314	WP_021193284.1	MFS transporter	-
ACS15_RS25195	1480231..1481469	-	1239	WP_021193283.1	cystathionine gamma-synthase family protein	-
ACS15_RS25200	1481750..1482046	+	297	WP_021193282.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACS15_RS25205	1482048..1482341	+	294	WP_021193281.1	putative addiction module antidote protein	Antitoxin
ACS15_RS25210	1482599..1485805	+	3207	WP_021193280.1	efflux RND transporter permease subunit	-
ACS15_RS25215	1485798..1487102	+	1305	WP_021193279.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11051.68 Da
• Isoelectric Point: 10.4496

>T56781 WP_021193282.1 NZ_CP012606:1481750-1482046 [Ralstonia insidiosa]
VIELKQTETFRKWRTKLKDERARAAIALRLARLGYGHVGDAEPVGQGISELRIHYGPGYRIYFHQRGSTIVILLCGGDKS
TQAKDINTAKRLAAQWSE

Nucleotide

• Length: 297 bp

>T56781 NZ_CP012606:1481750-1482046 [Ralstonia insidiosa]
GTGATCGAGCTCAAGCAAACCGAGACGTTCCGAAAGTGGCGCACAAAGCTCAAGGACGAGCGCGCTCGCGCTGCCATCGC
CTTGCGGTTGGCCCGTTTGGGTTATGGCCACGTTGGCGATGCCGAACCGGTCGGACAAGGCATTAGTGAGCTGCGCATCC
ACTACGGGCCTGGATATCGCATTTATTTCCATCAGCGAGGCAGCACGATCGTTATCCTGCTTTGCGGTGGTGATAAAAGC
ACGCAGGCCAAGGACATCAACACGGCCAAGCGCCTCGCTGCGCAATGGAGCGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10398.78 Da
• Isoelectric Point: 5.0216

>AT56781 WP_021193281.1 NZ_CP012606:1482048-1482341 [Ralstonia insidiosa]
MTDKLTTYDPAEDLTSDEAIAAFMAGAFETNDAGFISHALGIVARAKGMAQIANDTGLSREQLYRSFSEQGNPTLKTLLA
VMRALGIELTTKVHVAH

Nucleotide

• Length: 294 bp

>AT56781 NZ_CP012606:1482048-1482341 [Ralstonia insidiosa]
ATGACCGACAAGTTGACGACCTATGATCCGGCTGAGGATCTGACCTCTGACGAAGCCATCGCTGCGTTCATGGCCGGCGC
ATTCGAAACCAACGATGCTGGCTTCATCTCGCATGCGCTGGGAATCGTCGCCCGCGCCAAAGGCATGGCACAGATCGCCA
ATGACACCGGTCTTTCCCGCGAACAGCTCTATCGCTCATTCAGTGAGCAGGGCAATCCCACATTGAAGACCTTGCTGGCT
GTCATGCGAGCGCTCGGTATTGAATTGACGACCAAGGTCCACGTTGCCCACTGA

Structures

Toxin

• AlphaFold DB: A0A117DRY5