Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 3359496..3360124 Replicon chromosome
Accession NZ_CP012603
Organism Leptospira interrogans serovar Hardjo str. Norma isolate Cattle

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag G436_RS14425 Protein ID WP_000281042.1
Coordinates 3359726..3360124 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag G436_RS14420 Protein ID WP_001192482.1
Coordinates 3359496..3359726 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G436_RS23105 3355489..3355831 + 343 Protein_2772 tyrosine-type recombinase/integrase -
G436_RS14390 3355880..3356902 + 1023 WP_000288518.1 hypothetical protein -
G436_RS14395 3356957..3357484 + 528 WP_002188620.1 hypothetical protein -
G436_RS14400 3357763..3358098 + 336 WP_000669388.1 type II toxin-antitoxin system RelE/ParE family toxin -
G436_RS14405 3358095..3358394 + 300 WP_000769809.1 helix-turn-helix transcriptional regulator -
G436_RS14410 3358515..3359144 + 630 WP_000639168.1 hypothetical protein -
G436_RS14420 3359496..3359726 + 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G436_RS14425 3359726..3360124 + 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
G436_RS14430 3360222..3360770 + 549 WP_000479564.1 hypothetical protein -
G436_RS14435 3360912..3361988 + 1077 WP_001170569.1 hypothetical protein -
G436_RS14440 3361989..3362447 + 459 WP_060684617.1 GNAT family N-acetyltransferase -
G436_RS23110 3362664..3363526 - 863 Protein_2783 hypothetical protein -
G436_RS14450 3364037..3364804 - 768 WP_000636953.1 DUF1554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T56779 WP_000281042.1 NZ_CP012603:3359726-3360124 [Leptospira interrogans serovar Hardjo str. Norma]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T56779 NZ_CP012603:3359726-3360124 [Leptospira interrogans serovar Hardjo str. Norma]
ATGTATCTTTTGGATACCAATATATGCATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT56779 WP_001192482.1 NZ_CP012603:3359496-3359726 [Leptospira interrogans serovar Hardjo str. Norma]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT56779 NZ_CP012603:3359496-3359726 [Leptospira interrogans serovar Hardjo str. Norma]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTCAGATTACCAAAAGAATTTCAATTTACCGGAGATGA
TGTTCTCATTCAAAAAGTCGGAGAGGCGGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGAGAGAGAACAGCCGAAATCTGATAAAAGAGAAAAGCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References