Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1021760..1022336 Replicon chromosome
Accession NZ_CP012603
Organism Leptospira interrogans serovar Hardjo str. Norma isolate Cattle

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag G436_RS04555 Protein ID WP_000617906.1
Coordinates 1021995..1022336 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag G436_RS04550 Protein ID WP_000844758.1
Coordinates 1021760..1022008 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G436_RS04535 1017411..1019393 + 1983 WP_000836894.1 GAF domain-containing protein -
G436_RS23660 1020035..1020493 - 459 WP_000453267.1 hypothetical protein -
G436_RS04545 1020647..1020943 + 297 WP_000477061.1 helix-turn-helix domain-containing protein -
G436_RS04550 1021760..1022008 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
G436_RS04555 1021995..1022336 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
G436_RS04560 1023431..1023943 + 513 WP_000079224.1 VOC family protein -
G436_RS04565 1024627..1025220 + 594 WP_001226990.1 dihydrofolate reductase family protein -
G436_RS04570 1025292..1026179 + 888 WP_002188401.1 hypothetical protein -
G436_RS04575 1026297..1027043 + 747 WP_000617027.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T56778 WP_000617906.1 NZ_CP012603:1021995-1022336 [Leptospira interrogans serovar Hardjo str. Norma]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T56778 NZ_CP012603:1021995-1022336 [Leptospira interrogans serovar Hardjo str. Norma]
ATGATTCGTGGTGAAATTTGGTGGGTAGATTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTCTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT56778 WP_000844758.1 NZ_CP012603:1021760-1022008 [Leptospira interrogans serovar Hardjo str. Norma]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT56778 NZ_CP012603:1021760-1022008 [Leptospira interrogans serovar Hardjo str. Norma]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References