Detailed information of TA system TA056755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 23503..24091
• Replicon: chromosome
• Accession: NZ_CP012590
• Organism: Actinomyces sp. oral taxon 414 strain F0588

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0M3RJ07
• Locus tag: AM609_RS00120
• Protein ID: WP_053585634.1
• Coordinates: 23503..23853 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AM609_RS00125
• Protein ID: WP_053585635.1
• Coordinates: 23855..24091 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM609_RS00095	19137..19544	+	408	WP_083470490.1	GntR family transcriptional regulator	-
AM609_RS00100	19496..20449	+	954	WP_053585631.1	ABC transporter ATP-binding protein	-
AM609_RS00105	20488..21141	+	654	WP_053585632.1	ABC-2 transporter permease	-
AM609_RS15225	22327..22530	-	204	WP_083470491.1	integrase core domain-containing protein	-
AM609_RS16105	22930..23157	-	228	WP_083470739.1	IS3 family transposase	-
AM609_RS16110	23250..23498	-	249	WP_157065804.1	transposase	-
AM609_RS00120	23503..23853	-	351	WP_053585634.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AM609_RS00125	23855..24091	-	237	WP_053585635.1	hypothetical protein	Antitoxin
AM609_RS00130	24250..24753	+	504	WP_053585636.1	SMI1/KNR4 family protein	-
AM609_RS00135	24980..26146	-	1167	Protein_26	ISAs1 family transposase	-
AM609_RS00140	26345..27256	+	912	WP_083470946.1	ABC transporter ATP-binding protein/permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-93)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12401.38 Da
• Isoelectric Point: 8.5379

>T56755 WP_053585634.1 NZ_CP012590:c23853-23503 [Actinomyces sp. oral taxon 414]
VREVCLARLDKTRPVVILTRAAAREAMTKVTVAPITSTVKGLSSEVPVGPAEGLDHACAISLDNVLTIPSSRLGRTLGLL
SPAQEKALARAIVLAYDWSEWDPLDLGRVSSRDRIG

Nucleotide

• Length: 351 bp

>T56755 NZ_CP012590:c23853-23503 [Actinomyces sp. oral taxon 414]
GTGCGCGAGGTCTGCCTGGCCAGGCTCGACAAGACCCGCCCCGTCGTCATCCTCACCCGTGCAGCGGCACGCGAGGCGAT
GACGAAGGTGACGGTTGCGCCCATCACCTCGACCGTCAAGGGGCTCTCAAGCGAGGTCCCGGTGGGGCCCGCCGAGGGGC
TCGACCATGCCTGCGCGATCAGCCTCGACAACGTCCTGACGATCCCGAGCAGCCGCCTGGGCCGGACACTCGGCCTCCTC
AGCCCCGCGCAGGAGAAGGCCCTCGCCCGCGCGATCGTCCTCGCCTACGACTGGAGTGAGTGGGATCCTCTGGACTTGGG
GAGAGTTTCTTCCCGCGACAGAATAGGATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8603.60 Da
• Isoelectric Point: 4.2336

>AT56755 WP_053585635.1 NZ_CP012590:c24091-23855 [Actinomyces sp. oral taxon 414]
MSTQIAVRLPDDVVTFLDEAVSAGVAPSRASLVTEALEREMRRRAALRDLTILHREGPADDLDELVAWSTDQRSAPED

Nucleotide

• Length: 237 bp

>AT56755 NZ_CP012590:c24091-23855 [Actinomyces sp. oral taxon 414]
ATGAGCACCCAGATCGCCGTGAGGCTGCCCGACGACGTCGTCACCTTCCTCGACGAGGCCGTCTCCGCCGGGGTCGCTCC
GAGCCGGGCATCTCTCGTCACCGAGGCCCTTGAGCGCGAGATGCGCCGCCGGGCCGCGTTGCGCGACCTCACGATCCTGC
ACCGCGAGGGCCCCGCCGACGACCTCGATGAGCTCGTCGCCTGGAGCACCGACCAGCGTTCTGCCCCTGAGGACTGA

Structures

Toxin

• AlphaFold DB: A0A0M3RJ07