Detailed information of TA system TA056702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 285529..286066
• Replicon: chromosome
• Accession: NZ_CP012577
• Organism: Burkholderia pseudomallei strain 982

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: AMS56_RS19095
• Protein ID: WP_004549731.1
• Coordinates: 285797..286066 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: AMS56_RS19090
• Protein ID: WP_004525420.1
• Coordinates: 285529..285813 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMS56_RS19070	280637..281899	-	1263	WP_004551329.1	c-type cytochrome	-
AMS56_RS19075	282058..283518	-	1461	WP_011326543.1	metallophosphoesterase	-
AMS56_RS19080	283765..283881	+	117	Protein_214	IS3 family transposase	-
AMS56_RS34755	284108..284536	-	429	Protein_215	IS110 family transposase	-
AMS56_RS34760	284499..284882	-	384	Protein_216	DDE-type integrase/transposase/recombinase	-
AMS56_RS19090	285529..285813	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
AMS56_RS19095	285797..286066	-	270	WP_004549731.1	BrnT family toxin	Toxin
AMS56_RS19100	286637..286912	+	276	WP_038716556.1	hypothetical protein	-
AMS56_RS19105	286922..287053	+	132	WP_009923452.1	bacteriophage protein Gp48	-
AMS56_RS19115	287191..287433	+	243	WP_004545584.1	hypothetical protein	-
AMS56_RS39465	287430..287588	+	159	WP_004549802.1	hypothetical protein	-
AMS56_RS39470	287636..287854	+	219	WP_004552392.1	hypothetical protein	-
AMS56_RS19125	288085..288666	-	582	WP_004542548.1	hypothetical protein	-
AMS56_RS19130	288922..289071	+	150	WP_004542626.1	bacteriophage protein Gp44	-
AMS56_RS39475	289068..289841	+	774	Protein_226	prohibitin family protein	-
AMS56_RS39480	289839..290267	+	429	Protein_227	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	282058..292578	10520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T56702 WP_004549731.1 NZ_CP012577:c286066-285797 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T56702 NZ_CP012577:c286066-285797 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT56702 WP_004525420.1 NZ_CP012577:c285813-285529 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT56702 NZ_CP012577:c285813-285529 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: A0A0F6L9S8