Detailed information of TA system TA056701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 46243..46988
• Replicon: plasmid pUCLAOXA232-6.X
• Accession: NZ_CP012572
• Organism: Klebsiella pneumoniae strain UCLAOXA232KP

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: AM278_RS29850
• Protein ID: WP_032408901.1
• Coordinates: 46243..46734 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: AM278_RS29855
• Protein ID: WP_014386183.1
• Coordinates: 46722..46988 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM278_RS29820	41927..42682	+	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-
AM278_RS29825	42944..43384	+	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
AM278_RS29830	43381..43731	+	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
AM278_RS29840	43762..45354	+	1593	Protein_48	IS66 family transposase	-
AM278_RS29845	45551..45802	+	252	WP_032408900.1	aldo/keto reductase	-
AM278_RS29850	46243..46734	-	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
AM278_RS29855	46722..46988	-	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
AM278_RS29860	47185..47343	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
AM278_RS29865	47587..48072	-	486	WP_014386185.1	hypothetical protein	-
AM278_RS29870	48262..48534	+	273	WP_032408902.1	hypothetical protein	-
AM278_RS29875	48531..48881	+	351	WP_014386186.1	hypothetical protein	-
AM278_RS29890	49518..49844	+	327	WP_014386187.1	hypothetical protein	-
AM278_RS29895	49906..50118	+	213	WP_014386188.1	hypothetical protein	-
AM278_RS29900	50129..50353	+	225	WP_014386189.1	hypothetical protein	-
AM278_RS29905	50434..50754	+	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AM278_RS29910	50744..51022	+	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
AM278_RS29915	51023..51436	+	414	WP_013023817.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..163420	163420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T56701 WP_032408901.1 NZ_CP012572:c46734-46243 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T56701 NZ_CP012572:c46734-46243 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT56701 WP_014386183.1 NZ_CP012572:c46988-46722 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT56701 NZ_CP012572:c46988-46722 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6