Detailed information of TA system TA056698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 25753..26396
• Replicon: plasmid pUCLAOXA232-3.X
• Accession: NZ_CP012569
• Organism: Klebsiella pneumoniae strain UCLAOXA232KP

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: AM278_RS27730
• Protein ID: WP_071854281.1
• Coordinates: 25753..26169 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: AM278_RS27735
• Protein ID: WP_001261282.1
• Coordinates: 26166..26396 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM278_RS31995	21571..21636	-	66	Protein_23	EAL domain-containing protein	-
AM278_RS27705	21692..22906	+	1215	WP_004098817.1	type II site-specific deoxyribonuclease	-
AM278_RS27710	22940..24343	-	1404	WP_072094655.1	DNA cytosine methyltransferase	-
AM278_RS31345	24472..24657	+	186	Protein_26	IS1 family transposase	-
AM278_RS27720	24692..25396	-	705	WP_071854279.1	IS6-like element IS26 family transposase	-
AM278_RS27725	25443..25679	-	237	WP_001348075.1	AAA family ATPase	-
AM278_RS27730	25753..26169	-	417	WP_071854281.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM278_RS27735	26166..26396	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM278_RS27740	26353..26814	+	462	WP_165763053.1	hypothetical protein	-
AM278_RS27745	26959..27273	+	315	WP_015632378.1	hypothetical protein	-
AM278_RS27750	27322..27633	+	312	WP_024191724.1	hypothetical protein	-
AM278_RS27755	27698..28702	+	1005	WP_016162067.1	hypothetical protein	-
AM278_RS32000	28746..28907	+	162	WP_032495756.1	hypothetical protein	-
AM278_RS27760	28900..29694	+	795	WP_015632375.1	site-specific integrase	-
AM278_RS30745	30166..30345	-	180	WP_006788217.1	hypothetical protein	-
AM278_RS27775	30465..31091	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ARR-3 / rmtF	-	1..83730	83730

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15086.51 Da
• Isoelectric Point: 7.1084

>T56698 WP_071854281.1 NZ_CP012569:c26169-25753 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T56698 NZ_CP012569:c26169-25753 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTTGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCGGCCGGCGCCGTACTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT56698 WP_001261282.1 NZ_CP012569:c26396-26166 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT56698 NZ_CP012569:c26396-26166 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3