Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 143163..143899 Replicon plasmid pUCLAOXA232-6
Accession NZ_CP012567
Organism Klebsiella pneumoniae strain UCLAOXA232KP

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM277_RS30835 Protein ID WP_003026803.1
Coordinates 143163..143645 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM277_RS30840 Protein ID WP_003026799.1
Coordinates 143633..143899 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM277_RS30815 138800..139762 + 963 WP_004152113.1 M48 family metalloprotease -
AM277_RS32155 139850..141104 + 1255 Protein_152 IS3 family transposase -
AM277_RS30830 141605..142951 - 1347 WP_020314316.1 ISNCY family transposase -
AM277_RS30835 143163..143645 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM277_RS30840 143633..143899 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM277_RS30845 144075..144329 - 255 WP_004152108.1 hypothetical protein -
AM277_RS30850 144405..144662 - 258 WP_004152107.1 hypothetical protein -
AM277_RS30855 144711..144914 - 204 WP_004152106.1 hemolysin expression modulator Hha -
AM277_RS30860 144948..145316 - 369 WP_004152105.1 hypothetical protein -
AM277_RS30865 145360..145854 - 495 WP_004152104.1 DNA-binding protein -
AM277_RS30870 145885..146460 - 576 WP_004152103.1 hypothetical protein -
AM277_RS30875 146448..146717 - 270 WP_004152102.1 hypothetical protein -
AM277_RS30880 147075..147425 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM277_RS30885 147475..147837 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 - 1..196706 196706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T56680 WP_003026803.1 NZ_CP012567:c143645-143163 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T56680 NZ_CP012567:c143645-143163 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT56680 WP_003026799.1 NZ_CP012567:c143899-143633 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT56680 NZ_CP012567:c143899-143633 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References