Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 22449..23092 Replicon plasmid pUCLAOXA232-6
Accession NZ_CP012567
Organism Klebsiella pneumoniae strain UCLAOXA232KP

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag AM277_RS30045 Protein ID WP_014386165.1
Coordinates 22449..22865 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag AM277_RS30050 Protein ID WP_032408893.1
Coordinates 22862..23092 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM277_RS30015 17695..18126 + 432 WP_014386161.1 conjugation system SOS inhibitor PsiB -
AM277_RS30020 18123..18851 + 729 WP_014386162.1 plasmid SOS inhibition protein A -
AM277_RS30025 18848..19174 + 327 WP_004152639.1 hypothetical protein -
AM277_RS30030 19230..19604 + 375 WP_032408891.1 hypothetical protein -
AM277_RS30035 19814..20839 + 1026 WP_015632466.1 IS110 family transposase -
AM277_RS30040 21158..22126 - 969 WP_074422984.1 IS5 family transposase -
AM277_RS30045 22449..22865 - 417 WP_014386165.1 PIN domain-containing protein Toxin
AM277_RS30050 22862..23092 - 231 WP_032408893.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM277_RS30055 23412..23846 + 435 WP_000103648.1 RidA family protein -
AM277_RS30060 23851..24525 + 675 WP_014386166.1 DUF1028 domain-containing protein -
AM277_RS30065 24541..25698 + 1158 WP_032408895.1 acetylornithine deacetylase -
AM277_RS30070 25869..27016 + 1148 Protein_29 ABC transporter ATP-binding protein -
AM277_RS30075 27023..28018 + 996 WP_000611945.1 NAD(P)H-quinone oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 - 1..196706 196706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-130)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15127.44 Da        Isoelectric Point: 7.8841

>T56678 WP_014386165.1 NZ_CP012567:c22865-22449 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Download         Length: 417 bp

>T56678 NZ_CP012567:c22865-22449 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8854.89 Da        Isoelectric Point: 4.5112

>AT56678 WP_032408893.1 NZ_CP012567:c23092-22862 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Download         Length: 231 bp

>AT56678 NZ_CP012567:c23092-22862 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References