Detailed information of TA system TA056632

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3582387..3583066
• Replicon: chromosome
• Accession: NZ_CP012554
• Organism: Citrobacter portucalensis strain P10159

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: R8WLP2
• Locus tag: P10159_RS17010
• Protein ID: WP_006812012.1
• Coordinates: 3582725..3583066 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: R8WMM2
• Locus tag: P10159_RS17005
• Protein ID: WP_006812013.1
• Coordinates: 3582387..3582704 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P10159_RS16980	3578414..3578815	-	402	WP_031523086.1	VOC family protein	-
P10159_RS16985	3578829..3579572	-	744	WP_060683210.1	SDR family oxidoreductase	-
P10159_RS16990	3579709..3580596	+	888	WP_031523082.1	AraC family transcriptional regulator	-
P10159_RS25110	3580930..3581090	+	161	Protein_3371	DUF905 family protein	-
P10159_RS16995	3581193..3581645	+	453	WP_060683211.1	antirestriction protein	-
P10159_RS17000	3581661..3582137	+	477	WP_047355118.1	RadC family protein	-
P10159_RS25115	3582146..3582367	+	222	WP_001548171.1	DUF987 domain-containing protein	-
P10159_RS17005	3582387..3582704	+	318	WP_006812013.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
P10159_RS17010	3582725..3583066	+	342	WP_006812012.1	TA system toxin CbtA family protein	Toxin
P10159_RS17020	3583429..3583935	+	507	WP_008785849.1	G/U mismatch-specific DNA glycosylase	-
P10159_RS17025	3583981..3585828	-	1848	WP_003024699.1	RNA polymerase sigma factor RpoD	-
P10159_RS17030	3585993..3587738	-	1746	WP_003828467.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12989.07 Da
• Isoelectric Point: 10.4476

>T56632 WP_006812012.1 NZ_CP012554:3582725-3583066 [Citrobacter portucalensis]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T56632 NZ_CP012554:3582725-3583066 [Citrobacter portucalensis]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTAGCACCCGTGGCTGTCTGGCAAATGCTACTGAC
CCGCCTGCTGAAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGAATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTCGGTACGGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.45 Da
• Isoelectric Point: 7.0166

>AT56632 WP_006812013.1 NZ_CP012554:3582387-3582704 [Citrobacter portucalensis]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT56632 NZ_CP012554:3582387-3582704 [Citrobacter portucalensis]
ATGACCCACATCAACTGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAGGGTAACCGACT
GCACTATCTGGCTGACAGGGCCAGCATCACCGGCAGGTTCAGTGACACCGAATGCCGGAAGCTGGATGAAACATGCCCGC
ACTTTATCCGCCAGATGGAGTCGATGCTAACCACCGGTGAACTCAGTCCCCAACACGCCCACTGCGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGAGTTAA

Structures

Toxin

• AlphaFold DB: R8WLP2

Antitoxin

• AlphaFold DB: R8WMM2