Detailed information of TA system TA056630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3131274..3131862
• Replicon: chromosome
• Accession: NZ_CP012554
• Organism: Citrobacter portucalensis strain P10159

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: P10159_RS14930
• Protein ID: WP_060683076.1
• Coordinates: 3131545..3131862 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A5B0STC7
• Locus tag: P10159_RS14925
• Protein ID: WP_032942057.1
• Coordinates: 3131274..3131552 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P10159_RS14915	3128727..3130358	+	1632	WP_003826514.1	Na/Pi cotransporter family protein	-
P10159_RS14920	3130474..3131163	-	690	WP_060683075.1	dipeptidase PepE	-
P10159_RS14925	3131274..3131552	-	279	WP_032942057.1	putative addiction module antidote protein	Antitoxin
P10159_RS14930	3131545..3131862	-	318	WP_060683076.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
P10159_RS14935	3132126..3132677	-	552	WP_060683077.1	AAA family ATPase	-
P10159_RS14940	3132809..3133114	+	306	WP_060683078.1	hypothetical protein	-
P10159_RS14945	3133164..3133976	-	813	WP_060683079.1	shikimate 5-dehydrogenase	-
P10159_RS14950	3133978..3135201	-	1224	WP_060683080.1	L-sorbose 1-phosphate reductase	-
P10159_RS14955	3135270..3136094	-	825	WP_060683081.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-96)

Antitoxin

• Uncharacterized protein HI1420 (3-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11959.93 Da
• Isoelectric Point: 9.2299

>T56630 WP_060683076.1 NZ_CP012554:c3131862-3131545 [Citrobacter portucalensis]
IMEILQTMVFQRWEHNLRDRRAKTLIATRLFRLANGLAGDVKPVGEGVSEMRISYGPGYRIYFKQQGCRIIILLCGGDKS
SQANDITLAKMLARTLDSQEVFCHE

Nucleotide

• Length: 318 bp

>T56630 NZ_CP012554:c3131862-3131545 [Citrobacter portucalensis]
ATCATGGAAATTCTTCAGACAATGGTTTTCCAGCGTTGGGAGCACAACCTACGGGACAGGAGAGCTAAAACGCTCATTGC
TACACGCCTTTTTCGTCTGGCTAATGGTTTAGCTGGTGATGTTAAACCTGTTGGGGAAGGCGTCAGCGAAATGCGCATTT
CTTACGGTCCTGGCTACAGGATTTACTTTAAACAGCAAGGTTGCCGCATCATCATTTTGTTATGTGGGGGCGATAAAAGC
TCACAAGCCAACGATATTACTCTTGCAAAAATGCTCGCCCGTACACTGGATTCACAGGAGGTCTTCTGCCATGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9872.45 Da
• Isoelectric Point: 5.8233

>AT56630 WP_032942057.1 NZ_CP012554:c3131552-3131274 [Citrobacter portucalensis]
MNKLTHYDPATALVDDEEIAVFMADALETGDAAYIAKALGVVARAKGMANIAAETGLSREQLYRSFSEKGNPTLKTTLAV
MKVLGIELTLKK

Nucleotide

• Length: 279 bp

>AT56630 NZ_CP012554:c3131552-3131274 [Citrobacter portucalensis]
ATGAATAAACTTACTCACTACGATCCCGCGACTGCGCTGGTTGATGATGAAGAAATTGCCGTGTTTATGGCCGATGCCCT
CGAAACAGGCGATGCAGCATACATTGCAAAAGCACTGGGCGTGGTAGCTCGCGCTAAGGGAATGGCGAACATTGCGGCAG
AAACGGGATTATCCCGCGAGCAGCTTTATCGCTCTTTTAGTGAGAAAGGTAACCCGACGCTGAAAACCACCCTTGCAGTG
ATGAAGGTACTCGGCATTGAATTGACACTTAAAAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A5B0STC7