Detailed information of TA system TA056614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): AbkB-relB/BrnT-RelB
• Location: 6651..7132
• Replicon: plasmid pPspP2G3b
• Accession: NZ_CP012531
• Organism: Psychrobacter sp. P2G3

Toxin (Protein)

• Gene name: AbkB
• Uniprot ID: -
• Locus tag: AK823_RS13780
• Protein ID: WP_203226621.1
• Coordinates: 6848..7132 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1R4IGJ2
• Locus tag: AK823_RS13775
• Protein ID: WP_068330392.1
• Coordinates: 6651..6851 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AK823_RS13755	4504..4899	-	396	WP_068330402.1	hypothetical protein	-
AK823_RS13760	4982..5356	-	375	WP_068330404.1	hypothetical protein	-
AK823_RS13765	5817..6218	+	402	WP_068330405.1	hypothetical protein	-
AK823_RS13770	6286..6546	+	261	WP_068330407.1	hypothetical protein	-
AK823_RS13775	6651..6851	+	201	WP_068330392.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AK823_RS13780	6848..7132	+	285	WP_203226621.1	BrnT family toxin	Toxin
AK823_RS13785	7050..7415	+	366	WP_082785778.1	hypothetical protein	-
AK823_RS13790	7434..7943	-	510	WP_068330411.1	hypothetical protein	-
AK823_RS13795	8148..8480	+	333	WP_068330422.1	zinc ribbon domain-containing protein	-
AK823_RS13800	8512..8994	+	483	WP_068330413.1	hypothetical protein	-
AK823_RS13805	9127..9759	-	633	WP_068330415.1	RloB family protein	-
AK823_RS13810	9759..11111	-	1353	WP_068330417.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11297	11297

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-87)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10869.04 Da
• Isoelectric Point: 4.9211

>T56614 WP_203226621.1 NZ_CP012531:6848-7132 [Psychrobacter sp. P2G3]
MIEFEWDEQKDSSNQRKHGLSFEEAARVFFDPLCLRQQDRYENGEERWQALGAVNGVAVLLVAHTQRDSEGGEVIRIISA
RPATKAERRFYEQG

Nucleotide

• Length: 285 bp

>T56614 NZ_CP012531:6848-7132 [Psychrobacter sp. P2G3]
ATGATTGAATTTGAATGGGATGAACAGAAAGATAGCAGTAATCAACGCAAACATGGCTTATCCTTTGAAGAAGCAGCTCG
GGTATTTTTTGATCCTTTGTGTTTGCGTCAGCAAGACCGCTATGAAAATGGTGAAGAACGGTGGCAAGCGCTTGGTGCAG
TGAATGGGGTAGCCGTATTGCTGGTCGCTCATACTCAAAGAGATAGTGAGGGTGGTGAGGTTATTCGTATCATATCAGCA
CGACCTGCCACCAAGGCTGAAAGGAGATTTTATGAACAAGGTTAG

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7457.34 Da
• Isoelectric Point: 4.1313

>AT56614 WP_068330392.1 NZ_CP012531:6651-6851 [Psychrobacter sp. P2G3]
MSTTNYNIRLDQELKDKAFSVLEGYGLTPSQAIKLFLHQVAETNSIPLSFDYQTMPSTNMLEDGDQ

Nucleotide

• Length: 201 bp

>AT56614 NZ_CP012531:6651-6851 [Psychrobacter sp. P2G3]
ATGAGCACAACCAATTATAATATCCGTCTGGATCAAGAATTAAAAGACAAGGCATTTTCGGTGCTTGAGGGCTATGGTTT
AACGCCTTCGCAAGCGATTAAGTTATTTTTGCATCAGGTAGCAGAAACCAATAGCATACCGCTGTCTTTTGATTATCAGA
CAATGCCTAGCACCAATATGCTTGAAGACGGTGATCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1R4IGJ2