Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1943837..1944408 Replicon chromosome
Accession NZ_CP012522
Organism Enterococcus faecium strain 64/3 isolate human patient

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag AMR85_RS09490 Protein ID WP_002286801.1
Coordinates 1944067..1944408 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag AMR85_RS09485 Protein ID WP_002323011.1
Coordinates 1943837..1944067 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMR85_RS09460 1939203..1940534 + 1332 WP_002330022.1 FAD-containing oxidoreductase -
AMR85_RS09465 1940556..1941182 + 627 WP_002286816.1 cysteine hydrolase -
AMR85_RS09470 1941365..1941946 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
AMR85_RS09475 1942432..1943007 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
AMR85_RS09480 1943212..1943550 - 339 WP_002286804.1 hypothetical protein -
AMR85_RS09485 1943837..1944067 + 231 WP_002323011.1 hypothetical protein Antitoxin
AMR85_RS09490 1944067..1944408 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AMR85_RS09500 1944976..1946138 + 1163 WP_086956687.1 IS3 family transposase -
AMR85_RS09505 1946267..1947868 + 1602 Protein_1808 SpaH/EbpB family LPXTG-anchored major pilin -
AMR85_RS09510 1947942..1948835 + 894 WP_002286772.1 class C sortase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T56612 WP_002286801.1 NZ_CP012522:1944067-1944408 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T56612 NZ_CP012522:1944067-1944408 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT56612 WP_002323011.1 NZ_CP012522:1943837-1944067 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT56612 NZ_CP012522:1943837-1944067 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References