Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 1655690..1656219 Replicon chromosome
Accession NZ_CP012522
Organism Enterococcus faecium strain 64/3 isolate human patient

Toxin (Protein)


Gene name relE Uniprot ID C9B8W9
Locus tag AMR85_RS08165 Protein ID WP_002294198.1
Coordinates 1655953..1656219 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C9B8W8
Locus tag AMR85_RS08160 Protein ID WP_002294199.1
Coordinates 1655690..1655953 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMR85_RS08150 1653065..1654888 + 1824 WP_002294202.1 APC family permease -
AMR85_RS08155 1655030..1655215 - 186 WP_002294201.1 hypothetical protein -
AMR85_RS08160 1655690..1655953 + 264 WP_002294199.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AMR85_RS08165 1655953..1656219 + 267 WP_002294198.1 Txe/YoeB family addiction module toxin Toxin
AMR85_RS08170 1656420..1656908 + 489 WP_002294196.1 mannosyl-glycoprotein endo-beta-N-acetylglucosamidase -
AMR85_RS08175 1657450..1658121 - 672 WP_002319469.1 ABC transporter permease -
AMR85_RS08180 1658118..1659035 - 918 WP_002294193.1 osmoprotectant ABC transporter substrate-binding protein -
AMR85_RS08185 1659032..1659673 - 642 WP_002293405.1 ABC transporter permease -
AMR85_RS08190 1659677..1660852 - 1176 WP_002294192.1 betaine/proline/choline family ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(22-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10646.11 Da        Isoelectric Point: 9.9931

>T56611 WP_002294198.1 NZ_CP012522:1655953-1656219 [Enterococcus faecium]
MSNYTVAIKNSAKVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Download         Length: 267 bp

>T56611 NZ_CP012522:1655953-1656219 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10016.03 Da        Isoelectric Point: 4.1207

>AT56611 WP_002294199.1 NZ_CP012522:1655690-1655953 [Enterococcus faecium]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNID
DIDWEAL

Download         Length: 264 bp

>AT56611 NZ_CP012522:1655690-1655953 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FMA0

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZU84

References