Detailed information of TA system TA056608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 526951..527392
• Replicon: chromosome
• Accession: NZ_CP012518
• Organism: Burkholderia pseudomallei strain vgh16W

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: AM257_RS20235
• Protein ID: WP_009939402.1
• Coordinates: 526951..527124 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: AM257_RS20240
• Protein ID: WP_004525420.1
• Coordinates: 527108..527392 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM257_RS40825	522882..523256	-	375	Protein_425	DDE-type integrase/transposase/recombinase	-
AM257_RS20195	523254..524027	-	774	Protein_426	prohibitin family protein	-
AM257_RS20200	524024..524173	-	150	WP_004537646.1	bacteriophage protein Gp44	-
AM257_RS20205	524432..525013	+	582	WP_004542548.1	hypothetical protein	-
AM257_RS40355	525244..525543	-	300	WP_160458000.1	hypothetical protein	-
AM257_RS20215	525510..525731	-	222	WP_004552390.1	hypothetical protein	-
AM257_RS20225	525867..525998	-	132	WP_009923452.1	bacteriophage protein Gp48	-
AM257_RS20230	526008..526283	-	276	WP_009975384.1	hypothetical protein	-
AM257_RS20235	526951..527124	+	174	WP_009939402.1	BrnT family toxin	Toxin
AM257_RS20240	527108..527392	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
AM257_RS20245	528039..528422	+	384	Protein_435	DDE-type integrase/transposase/recombinase	-
AM257_RS35450	528385..528813	+	429	Protein_436	IS110 family transposase	-
AM257_RS20250	529040..529156	-	117	Protein_437	IS3 family transposase	-
AM257_RS20255	529460..530920	+	1461	WP_004547073.1	metallophosphoesterase	-
AM257_RS20260	531079..532341	+	1263	WP_004530738.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	518715..528422	9707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T56608 WP_009939402.1 NZ_CP012518:526951-527124 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T56608 NZ_CP012518:526951-527124 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT56608 WP_004525420.1 NZ_CP012518:527108-527392 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT56608 NZ_CP012518:527108-527392 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8