Detailed information of TA system TA056605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 526950..527391
• Replicon: chromosome
• Accession: NZ_CP012516
• Organism: Burkholderia pseudomallei strain vgh16R

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: AM256_RS20215
• Protein ID: WP_009939402.1
• Coordinates: 526950..527123 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: AM256_RS20220
• Protein ID: WP_004525420.1
• Coordinates: 527107..527391 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM256_RS40845	522882..523256	-	375	Protein_426	DDE-type integrase/transposase/recombinase	-
AM256_RS20175	523254..524027	-	774	Protein_427	prohibitin family protein	-
AM256_RS40365	523963..524331	-	369	WP_162491312.1	hypothetical protein	-
AM256_RS20185	524431..525012	+	582	WP_004542548.1	hypothetical protein	-
AM256_RS40370	525243..525542	-	300	WP_160458000.1	hypothetical protein	-
AM256_RS20195	525509..525730	-	222	WP_004552390.1	hypothetical protein	-
AM256_RS20205	525866..525997	-	132	WP_009923452.1	bacteriophage protein Gp48	-
AM256_RS20210	526007..526282	-	276	WP_009975384.1	hypothetical protein	-
AM256_RS20215	526950..527123	+	174	WP_009939402.1	BrnT family toxin	Toxin
AM256_RS20220	527107..527391	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
AM256_RS20225	528038..528421	+	384	Protein_436	DDE-type integrase/transposase/recombinase	-
AM256_RS35420	528384..528812	+	429	Protein_437	IS110 family transposase	-
AM256_RS20230	529039..529155	-	117	Protein_438	IS3 family transposase	-
AM256_RS20235	529459..530919	+	1461	WP_004547073.1	metallophosphoesterase	-
AM256_RS20240	531078..532340	+	1263	WP_004530738.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	518714..528421	9707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T56605 WP_009939402.1 NZ_CP012516:526950-527123 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T56605 NZ_CP012516:526950-527123 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT56605 WP_004525420.1 NZ_CP012516:527107-527391 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT56605 NZ_CP012516:527107-527391 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8