Detailed information of TA system TA056598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3827089..3827774
• Replicon: chromosome
• Accession: NZ_CP012506
• Organism: Mycobacterium tuberculosis strain SCAID 187.0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: AFL40_RS18005
• Protein ID: WP_003417998.1
• Coordinates: 3827364..3827774 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AFL40_RS18000
• Protein ID: WP_180895138.1
• Coordinates: 3827089..3827367 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFL40_RS17980	3823078..3824679	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
AFL40_RS17985	3824696..3825400	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
AFL40_RS17990	3825518..3826084	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
AFL40_RS17995	3826146..3827033	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
AFL40_RS18000	3827089..3827367	+	279	WP_180895138.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AFL40_RS18005	3827364..3827774	+	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
AFL40_RS18010	3827807..3829543	-	1737	WP_003418002.1	cholesterol oxidase	-
AFL40_RS18015	3829599..3830726	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
AFL40_RS18020	3830746..3832335	-	1590	WP_003901630.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-58)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T56598 WP_003417998.1 NZ_CP012506:3827364-3827774 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T56598 NZ_CP012506:3827364-3827774 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10227.70 Da
• Isoelectric Point: 8.0222

>AT56598 WP_180895138.1 NZ_CP012506:3827089-3827367 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGCKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT56598 NZ_CP012506:3827089-3827367 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCTGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: G0TIR9