Detailed information of TA system TA056588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3065652..3066339
• Replicon: chromosome
• Accession: NZ_CP012506
• Organism: Mycobacterium tuberculosis strain SCAID 187.0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: AFL40_RS14535
• Protein ID: WP_003414064.1
• Coordinates: 3065944..3066339 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: AFL40_RS14530
• Protein ID: WP_003414061.1
• Coordinates: 3065652..3065918 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFL40_RS14505	3061291..3062193	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AFL40_RS14510	3062262..3063014	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
AFL40_RS14515	3063258..3063533	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
AFL40_RS14520	3063530..3065152	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
AFL40_RS14525	3065239..3065655	-	417	WP_059396479.1	type II toxin-antitoxin system toxin ribonuclease C21	-
AFL40_RS14530	3065652..3065918	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AFL40_RS14535	3065944..3066339	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
AFL40_RS14540	3066336..3066605	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
AFL40_RS14545	3066615..3067709	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
AFL40_RS14550	3067706..3068125	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
AFL40_RS14555	3068199..3068678	-	480	WP_003414073.1	dihydrofolate reductase	-
AFL40_RS14560	3068749..3069549	-	801	WP_003911953.1	thymidylate synthase	-
AFL40_RS14565	3069705..3070442	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T56588 WP_003414064.1 NZ_CP012506:c3066339-3065944 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T56588 NZ_CP012506:c3066339-3065944 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT56588 WP_003414061.1 NZ_CP012506:c3065918-3065652 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT56588 NZ_CP012506:c3065918-3065652 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64