Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2864751..2865388 Replicon chromosome
Accession NZ_CP012506
Organism Mycobacterium tuberculosis strain SCAID 187.0

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag AFL40_RS13455 Protein ID WP_003413180.1
Coordinates 2864751..2865146 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag AFL40_RS13460 Protein ID WP_003413183.1
Coordinates 2865143..2865388 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFL40_RS13420 2860155..2861366 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
AFL40_RS13425 2861493..2862152 + 660 WP_003902296.1 hypothetical protein -
AFL40_RS13430 2862149..2862811 + 663 WP_003905878.1 hypothetical protein -
AFL40_RS22170 2862808..2863085 + 278 Protein_2621 type II toxin-antitoxin system VapB family antitoxin -
AFL40_RS13435 2863178..2863591 + 414 WP_003413164.1 PIN domain nuclease -
AFL40_RS13440 2863630..2863887 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
AFL40_RS13445 2863884..2864261 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
AFL40_RS13450 2864277..2864651 - 375 WP_003413177.1 hypothetical protein -
AFL40_RS13455 2864751..2865146 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
AFL40_RS13460 2865143..2865388 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
AFL40_RS13465 2865799..2866218 - 420 WP_003413190.1 A24 family peptidase -
AFL40_RS13470 2866230..2867039 - 810 WP_003413193.1 shikimate dehydrogenase -
AFL40_RS13475 2867036..2868289 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
AFL40_RS13480 2868282..2868794 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T56584 WP_003413180.1 NZ_CP012506:c2865146-2864751 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T56584 NZ_CP012506:c2865146-2864751 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT56584 WP_003413183.1 NZ_CP012506:c2865388-2865143 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT56584 NZ_CP012506:c2865388-2865143 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References