Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2803113..2803765 Replicon chromosome
Accession NZ_CP012506
Organism Mycobacterium tuberculosis strain SCAID 187.0

Toxin (Protein)


Gene name vapC Uniprot ID A0A5R1ZCY8
Locus tag AFL40_RS13155 Protein ID WP_003905869.1
Coordinates 2803340..2803765 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag AFL40_RS13150 Protein ID WP_003412749.1
Coordinates 2803113..2803334 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFL40_RS13135 2801353..2801559 - 207 WP_003899347.1 hypothetical protein -
AFL40_RS13140 2801695..2802318 + 624 WP_003900858.1 TIGR00725 family protein -
AFL40_RS13145 2802308..2803060 + 753 WP_003901416.1 hypothetical protein -
AFL40_RS13150 2803113..2803334 + 222 WP_003412749.1 antitoxin Antitoxin
AFL40_RS13155 2803340..2803765 + 426 WP_003905869.1 type II toxin-antitoxin system VapC family toxin Toxin
AFL40_RS13160 2803788..2804969 - 1182 WP_003905870.1 2-oxo acid dehydrogenase subunit E2 -
AFL40_RS13165 2804966..2806012 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
AFL40_RS13170 2806023..2807126 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
AFL40_RS13175 2807385..2808206 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
AFL40_RS13180 2808203..2808760 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15364.84 Da        Isoelectric Point: 9.2386

>T56580 WP_003905869.1 NZ_CP012506:2803340-2803765 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRASTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T56580 NZ_CP012506:2803340-2803765 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGCGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT56580 WP_003412749.1 NZ_CP012506:2803113-2803334 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT56580 NZ_CP012506:2803113-2803334 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5R1ZCY8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References