Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2316676..2317312 Replicon chromosome
Accession NZ_CP012506
Organism Mycobacterium tuberculosis strain SCAID 187.0

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag AFL40_RS10810 Protein ID WP_031664648.1
Coordinates 2316902..2317312 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag AFL40_RS10805 Protein ID WP_003410651.1
Coordinates 2316676..2316909 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFL40_RS10795 2312526..2312930 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
AFL40_RS10800 2313014..2316598 - 3585 WP_003910889.1 cobaltochelatase subunit CobN -
AFL40_RS10805 2316676..2316909 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
AFL40_RS10810 2316902..2317312 + 411 WP_031664648.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
AFL40_RS10815 2317296..2318387 + 1092 WP_003900454.1 precorrin-3B synthase -
AFL40_RS10820 2318397..2319023 + 627 WP_003410658.1 precorrin-8X methylmutase -
AFL40_RS10825 2319020..2320546 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
AFL40_RS10830 2320492..2321715 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14236.40 Da        Isoelectric Point: 9.4815

>T56577 WP_031664648.1 NZ_CP012506:2316902-2317312 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIEDALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T56577 NZ_CP012506:2316902-2317312 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAGACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT56577 WP_003410651.1 NZ_CP012506:2316676-2316909 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT56577 NZ_CP012506:2316676-2316909 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References