Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2214902..2215590 Replicon chromosome
Accession NZ_CP012506
Organism Mycobacterium tuberculosis strain SCAID 187.0

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag AFL40_RS10340 Protein ID WP_003409958.1
Coordinates 2214902..2215321 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag AFL40_RS10345 Protein ID WP_003409968.1
Coordinates 2215330..2215590 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFL40_RS10320 2210397..2211245 + 849 WP_003901306.1 class I SAM-dependent methyltransferase -
AFL40_RS10325 2211208..2212653 - 1446 WP_003899115.1 APC family permease -
AFL40_RS10330 2212832..2213518 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
AFL40_RS10335 2213709..2214677 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
AFL40_RS10340 2214902..2215321 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
AFL40_RS10345 2215330..2215590 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
AFL40_RS10350 2215733..2217409 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
AFL40_RS10355 2217397..2218050 - 654 WP_003409976.1 cutinase Cfp21 -
AFL40_RS10360 2218165..2218395 + 231 WP_003409980.1 DUF167 domain-containing protein -
AFL40_RS10365 2218480..2219391 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
AFL40_RS10370 2219500..2220099 + 600 WP_003910883.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T56572 WP_003409958.1 NZ_CP012506:c2215321-2214902 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T56572 NZ_CP012506:c2215321-2214902 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT56572 WP_003409968.1 NZ_CP012506:c2215590-2215330 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT56572 NZ_CP012506:c2215590-2215330 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References