Detailed information of TA system TA056569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2179642..2180345
• Replicon: chromosome
• Accession: NZ_CP012506
• Organism: Mycobacterium tuberculosis strain SCAID 187.0

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: AFL40_RS10095
• Protein ID: WP_003409778.1
• Coordinates: 2179642..2179971 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: AFL40_RS21255
• Protein ID: WP_003409780.1
• Coordinates: 2179968..2180345 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFL40_RS10075	2176025..2177095	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
AFL40_RS10080	2177092..2177607	+	516	WP_003409718.1	flavin reductase family protein	-
AFL40_RS10085	2177604..2178665	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
AFL40_RS10090	2178662..2179432	+	771	WP_003409775.1	SDR family oxidoreductase	-
AFL40_RS10095	2179642..2179971	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AFL40_RS21255	2179968..2180345	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
AFL40_RS10105	2180342..2180932	-	591	WP_003409784.1	SEC-C domain-containing protein	-
AFL40_RS10110	2180987..2182351	+	1365	WP_003903691.1	HNH endonuclease	-
AFL40_RS10115	2182506..2182958	-	453	WP_031664647.1	hypothetical protein	-
AFL40_RS10120	2183022..2183423	+	402	WP_003409869.1	hypothetical protein	-
AFL40_RS10125	2183416..2183598	-	183	WP_003409870.1	hypothetical protein	-
AFL40_RS10130	2183712..2184062	-	351	WP_003409871.1	hypothetical protein	-
AFL40_RS10135	2184073..2184975	-	903	WP_003899097.1	hypothetical protein	-
AFL40_RS10140	2184996..2185187	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T56569 WP_003409778.1 NZ_CP012506:c2179971-2179642 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T56569 NZ_CP012506:c2179971-2179642 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT56569 WP_003409780.1 NZ_CP012506:c2180345-2179968 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT56569 NZ_CP012506:c2180345-2179968 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8