Detailed information of TA system TA056560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1235006..1235574
• Replicon: chromosome
• Accession: NZ_CP012506
• Organism: Mycobacterium tuberculosis strain SCAID 187.0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: AFL40_RS05865
• Protein ID: WP_003405865.1
• Coordinates: 1235200..1235574 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: AFL40_RS05860
• Protein ID: WP_003405863.1
• Coordinates: 1235006..1235203 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFL40_RS05840	1231047..1231685	-	639	WP_003898730.1	lipid droplet-associated protein	-
AFL40_RS05845	1231775..1232782	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
AFL40_RS05850	1232799..1233782	-	984	WP_003898731.1	hypothetical protein	-
AFL40_RS05855	1233845..1234918	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
AFL40_RS05860	1235006..1235203	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AFL40_RS05865	1235200..1235574	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
AFL40_RS05870	1235777..1236475	+	699	WP_003898733.1	hypothetical protein	-
AFL40_RS05875	1236593..1236778	+	186	WP_003901093.1	hypothetical protein	-
AFL40_RS05880	1236705..1236980	-	276	WP_003405867.1	hypothetical protein	-
AFL40_RS05885	1237223..1237546	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
AFL40_RS05890	1237561..1238271	-	711	WP_003911399.1	hypothetical protein	-
AFL40_RS22480	1238293..1238502	+	210	WP_003911400.1	hypothetical protein	-
AFL40_RS05900	1238454..1239161	-	708	Protein_1155	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T56560 WP_003405865.1 NZ_CP012506:1235200-1235574 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T56560 NZ_CP012506:1235200-1235574 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT56560 WP_003405863.1 NZ_CP012506:1235006-1235203 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT56560 NZ_CP012506:1235006-1235203 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07