Detailed information of TA system TA056543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 442508..443519
• Replicon: chromosome
• Accession: NZ_CP012506
• Organism: Mycobacterium tuberculosis strain SCAID 187.0

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: L7N5R2
• Locus tag: AFL40_RS01895
• Protein ID: WP_003401859.1
• Coordinates: 442508..443101 (-)
• Length: 198 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: O53702
• Locus tag: AFL40_RS01900
• Protein ID: WP_003401860.1
• Coordinates: 443130..443519 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFL40_RS01875	438157..439539	+	1383	WP_003898415.1	magnesium transporter	-
AFL40_RS01880	439551..440585	-	1035	WP_003401856.1	class II fructose-bisphosphate aldolase	-
AFL40_RS01885	440681..441364	+	684	WP_003401857.1	VTT domain-containing protein	-
AFL40_RS01890	441353..442483	-	1131	WP_003401858.1	hypothetical protein	-
AFL40_RS01895	442508..443101	-	594	WP_003401859.1	zeta toxin family protein	Toxin
AFL40_RS01900	443130..443519	-	390	WP_003401860.1	hypothetical protein	Antitoxin
AFL40_RS01905	443600..444811	-	1212	WP_003401861.1	VWA domain-containing protein	-
AFL40_RS01910	444817..445419	-	603	WP_003898417.1	SRPBCC family protein	-
AFL40_RS01915	445433..446329	-	897	WP_003898418.1	MoxR family ATPase	-
AFL40_RS01920	446326..446919	-	594	WP_003401864.1	NTP transferase domain-containing protein	-
AFL40_RS01925	446916..447671	-	756	WP_025096343.1	XdhC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-156)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 198 a.a.
• Molecular weight: 21344.53 Da
• Isoelectric Point: 10.5018

>T56543 WP_003401859.1 NZ_CP012506:c443101-442508 [Mycobacterium tuberculosis]
VKRLDLVAGPNGAGKSTFVALTLAPLLPGIVFVNADEIAKQRWPDDPTSHAYQAAQVAADTRARLIDLGRPFIAETVFSH
PSKLELIRTARTAGYTVVLHVLVIPEGLAVERVRHRVAAGGHDVPETKIRERHRRLAELVAQAITLADGATVYDNSRLAG
PRIVAQFSGGGIIGRACWPSWTPPPLMSRWSNRPETA

Nucleotide

• Length: 594 bp

>T56543 NZ_CP012506:c443101-442508 [Mycobacterium tuberculosis]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13648.21 Da
• Isoelectric Point: 6.0829

>AT56543 WP_003401860.1 NZ_CP012506:c443519-443130 [Mycobacterium tuberculosis]
VPKAVDRVTRVAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSNQHTASRRRVEAALAGHLPMTDLTLEEGVVFNAEI
SAAIEERLSRTNYGDVLAAQGITTVALNDAGDIVEHRPDGTSVVLAATP

Nucleotide

• Length: 390 bp

>AT56543 NZ_CP012506:c443519-443130 [Mycobacterium tuberculosis]
GTGCCCAAAGCCGTCGACCGCGTCACGCGCGTCGCCGCGGATCTGGTGGACAGCGCGGCGGCCGAGGGTGCTCGTCAGAG
CCGTTCGGCCAAGCAGCAGCTCGACCACTGGGCCCGCGTCGGTCGGGCGGTGTCGAACCAGCACACGGCGTCGCGGCGCC
GCGTGGAAGCTGCGCTCGCGGGGCATCTGCCGATGACGGACCTGACGCTGGAGGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCAGCTATCGAGGAGCGCCTGTCGCGAACCAACTACGGCGACGTGCTCGCCGCGCAGGGCATCACCACGGTCGCCCT
GAACGACGCGGGCGACATCGTCGAGCACCGGCCAGACGGCACCTCGGTCGTGCTGGCGGCCACGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BRU8

Antitoxin

• AlphaFold DB: A0A7U4BRT0