Detailed information of TA system TA056536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 3006197..3006829
• Replicon: chromosome
• Accession: NZ_CP012502
• Organism: Bacillus beveridgei strain MLTeJB

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: BBEV_RS14110
• Protein ID: WP_069366044.1
• Coordinates: 3006197..3006547 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1D7QYQ8
• Locus tag: BBEV_RS14115
• Protein ID: WP_069366045.1
• Coordinates: 3006551..3006829 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBEV_RS14075	3001741..3002547	-	807	WP_084007410.1	RNA polymerase sigma factor SigB	-
BBEV_RS14080	3002510..3003010	-	501	WP_069366038.1	anti-sigma B factor RsbW	-
BBEV_RS14085	3003012..3003341	-	330	WP_069366039.1	STAS domain-containing protein	-
BBEV_RS14090	3003406..3004389	-	984	WP_198155096.1	PP2C family protein-serine/threonine phosphatase	-
BBEV_RS14095	3004448..3004849	-	402	WP_069366041.1	anti-sigma regulatory factor	-
BBEV_RS14100	3004854..3005234	-	381	WP_198155013.1	STAS domain-containing protein	-
BBEV_RS14105	3005191..3006051	-	861	WP_069366043.1	STAS domain-containing protein	-
BBEV_RS14110	3006197..3006547	-	351	WP_069366044.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BBEV_RS14115	3006551..3006829	-	279	WP_069366045.1	antitoxin	Antitoxin
BBEV_RS14120	3006959..3008131	-	1173	WP_069366766.1	alanine racemase	-
BBEV_RS14125	3008194..3009756	-	1563	WP_069366046.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
BBEV_RS14130	3009789..3010160	-	372	WP_069366047.1	holo-ACP synthase	-
BBEV_RS14135	3010269..3011033	+	765	WP_069366048.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13064.22 Da
• Isoelectric Point: 7.0142

>T56536 WP_069366044.1 NZ_CP012502:c3006547-3006197 [Bacillus beveridgei]
MMVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYRFDRDSVILLEQI
RTIDKQRLTDKITQLDQDMMEKVDDALRVSIGLIEL

Nucleotide

• Length: 351 bp

>T56536 NZ_CP012502:c3006547-3006197 [Bacillus beveridgei]
TTGATGGTAAAGCGAGGCGATGTCTATTTCGCCGATTTGTCACCGGTTGTCGGCTCCGAACAGGGAGGCGTGAGACCGGT
GCTTATTATTCAAAATGATATTGGGAACCGGTTCAGCCCGACAGTAATTGTCGCTGCGATTACTGCACAGATTCAAAAAG
CCAAACTTCCCACTCACGTGGAAATCAATGCAAAGAAATACAGGTTTGATCGTGATTCGGTTATCTTACTGGAACAAATC
CGCACGATTGACAAGCAGAGGCTCACGGACAAAATTACGCAGCTTGATCAGGATATGATGGAAAAAGTAGATGATGCACT
CCGGGTCAGCATTGGCCTGATTGAACTATAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10632.94 Da
• Isoelectric Point: 4.3356

>AT56536 WP_069366045.1 NZ_CP012502:c3006829-3006551 [Bacillus beveridgei]
VSTDYTNQISINLPQNLIQEIDALTEGEGCNRDELIHRELVRFVQLQRNKQIREEMQRGYKEMAKINLNIATESFLVEEE
AENALDRLVSGV

Nucleotide

• Length: 279 bp

>AT56536 NZ_CP012502:c3006829-3006551 [Bacillus beveridgei]
GTGTCAACAGATTACACGAATCAAATCAGCATTAATCTGCCACAAAATCTCATTCAAGAAATTGATGCATTAACCGAAGG
GGAAGGATGTAATCGGGACGAACTGATCCACCGTGAACTGGTAAGATTCGTTCAGTTACAGCGCAACAAGCAGATCCGCG
AAGAGATGCAGCGAGGGTATAAGGAAATGGCAAAAATCAACCTGAACATTGCAACGGAATCCTTTCTGGTTGAAGAGGAG
GCCGAGAACGCGCTGGACCGTCTGGTCAGCGGGGTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1D7QYQ8