Detailed information of TA system TA056535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 231937..232562
• Replicon: plasmid pCFSAN004179G
• Accession: NZ_CP012501
• Organism: Escherichia coli strain 08-00022

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0B0VN52
• Locus tag: CP48_RS01395
• Protein ID: WP_040090742.1
• Coordinates: 231937..232335 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: CP48_RS01400
• Protein ID: WP_000450526.1
• Coordinates: 232335..232562 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP48_RS01365	227254..227751	+	498	WP_040090738.1	entry exclusion protein	-
CP48_RS01370	227783..228514	+	732	WP_040090739.1	complement resistance protein TraT	-
CP48_RS01375	228574..228834	+	261	WP_040090733.1	hypothetical protein	-
CP48_RS30265	229183..229719	+	537	WP_170919319.1	hypothetical protein	-
CP48_RS01390	229775..231928	+	2154	WP_047088736.1	type IV conjugative transfer system coupling protein TraD	-
CP48_RS01395	231937..232335	-	399	WP_040090742.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CP48_RS01400	232335..232562	-	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa / faeC / faeD / faeE / faeF / faeH / faeI / faeJ / stcE / exeE / exeG / hlyD / hlyB / hlyA / hlyC / estIa / estIa	1..242187	242187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14862.13 Da
• Isoelectric Point: 7.1084

>T56535 WP_040090742.1 NZ_CP012501:c232335-231937 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGCPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T56535 NZ_CP012501:c232335-231937 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACAATAAAGAACAAGCCCGCCAGTGTCAGAGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGATGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGCGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT56535 WP_000450526.1 NZ_CP012501:c232562-232335 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT56535 NZ_CP012501:c232562-232335 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGGCTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0B0VN52