Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 179055..179614 Replicon plasmid pCFSAN004179G
Accession NZ_CP012501
Organism Escherichia coli strain 08-00022

Toxin (Protein)


Gene name relE Uniprot ID A0A0B1JMW5
Locus tag CP48_RS01030 Protein ID WP_000421249.1
Coordinates 179339..179614 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0B1JM87
Locus tag CP48_RS01025 Protein ID WP_001355337.1
Coordinates 179055..179339 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP48_RS31845 175925..176101 - 177 WP_157839835.1 hypothetical protein -
CP48_RS30235 176407..177179 - 773 Protein_196 IS21-like element helper ATPase IstB -
CP48_RS01015 177176..178176 - 1001 Protein_197 IS21 family transposase -
CP48_RS28105 178190..178674 + 485 Protein_198 IS3 family transposase -
CP48_RS01025 179055..179339 + 285 WP_001355337.1 ribbon-helix-helix protein, CopG family Antitoxin
CP48_RS01030 179339..179614 + 276 WP_000421249.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CP48_RS01035 179720..180001 + 282 WP_001105063.1 hypothetical protein -
CP48_RS01040 180227..180700 - 474 Protein_202 hypothetical protein -
CP48_RS01045 180807..181157 - 351 WP_024166183.1 plasmid partitioning/stability family protein -
CP48_RS01050 181160..182122 - 963 WP_000959884.1 plasmid segregation protein ParM -
CP48_RS30240 182165..182466 + 302 Protein_205 hypothetical protein -
CP48_RS01060 182545..183228 + 684 WP_000085877.1 DNA methylase -
CP48_RS01065 183229..183450 + 222 WP_001104895.1 hypothetical protein -
CP48_RS01070 183446..183873 + 428 Protein_208 DUF1380 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa / faeC / faeD / faeE / faeF / faeH / faeI / faeJ / stcE / exeE / exeG / hlyD / hlyB / hlyA / hlyC / estIa / estIa 1..242187 242187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10722.42 Da        Isoelectric Point: 9.9324

>T56530 WP_000421249.1 NZ_CP012501:179339-179614 [Escherichia coli]
MELKWTSKALSDLARLYDFLALASKPAAARTVQSLTQAPIILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T56530 NZ_CP012501:179339-179614 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGCGCTGGCCAGTAAACCAGC
TGCAGCCAGAACGGTGCAGTCCCTGACACAGGCACCGATCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGTGAGTACGAAATCCGTTACGAACTTACTGGTCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10550.99 Da        Isoelectric Point: 5.6706

>AT56530 WP_001355337.1 NZ_CP012501:179055-179339 [Escherichia coli]
MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWIIKQALSAWIAQEEERNRLTLEALDDVTSGRVIDHQAVQAW
ADSLDTDNSLPVPR

Download         Length: 285 bp

>AT56530 NZ_CP012501:179055-179339 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGGTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGATTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCACTGGACGATGTGACATCCGGACGGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGATACTGACAATTCGTTACCGGTGCCTCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B1JMW5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B1JM87

References