Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 128526..129051 Replicon plasmid pCFSAN004179G
Accession NZ_CP012501
Organism Escherichia coli strain 08-00022

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CP48_RS00795 Protein ID WP_001159871.1
Coordinates 128526..128831 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0B0VNF4
Locus tag CP48_RS00800 Protein ID WP_000813635.1
Coordinates 128833..129051 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP48_RS31095 124416..124637 + 222 Protein_143 transposase -
CP48_RS00770 124697..125113 - 417 WP_001278817.1 plasmid partitioning/stability family protein -
CP48_RS00775 125106..126086 - 981 WP_000688520.1 plasmid segregation protein ParM -
CP48_RS00780 126500..126808 - 309 WP_000030205.1 hypothetical protein -
CP48_RS00785 126898..127539 - 642 WP_001144033.1 ParA family protein -
CP48_RS00790 127719..128525 - 807 WP_000016974.1 site-specific integrase -
CP48_RS00795 128526..128831 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CP48_RS00800 128833..129051 - 219 WP_000813635.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CP48_RS31825 129674..130195 - 522 Protein_151 MarR family transcriptional regulator -
CP48_RS00815 130318..131757 - 1440 WP_040090858.1 enterohemolysin T1SS ABC transporter subunit EhxD -
CP48_RS00820 131761..133881 - 2121 WP_072097151.1 enterohemolysin T1SS ABC transporter permease/ATPase EhxB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa / faeC / faeD / faeE / faeF / faeH / faeI / faeJ / stcE / exeE / exeG / hlyD / hlyB / hlyA / hlyC / estIa / estIa 1..242187 242187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T56529 WP_001159871.1 NZ_CP012501:c128831-128526 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T56529 NZ_CP012501:c128831-128526 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTAGCCAGCGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTCATTGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8386.37 Da        Isoelectric Point: 4.7558

>AT56529 WP_000813635.1 NZ_CP012501:c129051-128833 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENREW

Download         Length: 219 bp

>AT56529 NZ_CP012501:c129051-128833 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACAGTCGACAGTGACAGCTATCAGTTGCTCAAAGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VNF4

References