Detailed information of TA system TA056522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 100765..101324
• Replicon: plasmid pCFSAN004180G
• Accession: NZ_CP012500
• Organism: Escherichia coli strain 09-00049

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A193MI35
• Locus tag: GJ12_RS00635
• Protein ID: WP_000421271.1
• Coordinates: 100765..101040 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0B1JM87
• Locus tag: GJ12_RS00640
• Protein ID: WP_001355337.1
• Coordinates: 101040..101324 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ12_RS00595	96413..96840	-	428	Protein_114	DUF1380 family protein	-
GJ12_RS00600	96836..97057	-	222	WP_001104895.1	hypothetical protein	-
GJ12_RS00605	97058..97741	-	684	WP_000085877.1	DNA methylase	-
GJ12_RS30175	97820..98121	-	302	Protein_117	hypothetical protein	-
GJ12_RS00615	98259..99221	+	963	WP_000959884.1	plasmid segregation protein ParM	-
GJ12_RS00620	99224..99574	+	351	WP_024166183.1	plasmid partitioning/stability family protein	-
GJ12_RS00625	99681..100142	+	462	Protein_120	hypothetical protein	-
GJ12_RS00630	100378..100659	-	282	WP_001105063.1	hypothetical protein	-
GJ12_RS00635	100765..101040	-	276	WP_000421271.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJ12_RS00640	101040..101324	-	285	WP_001355337.1	ribbon-helix-helix protein, CopG family	Antitoxin
GJ12_RS00645	101717..101938	-	222	WP_001261037.1	MarR family transcriptional regulator	-
GJ12_RS28240	102045..102450	+	406	Protein_125	hypothetical protein	-
GJ12_RS30940	102439..102708	+	270	Protein_126	integrase core domain-containing protein	-
GJ12_RS00655	102824..103414	+	591	WP_001355339.1	hypothetical protein	-
GJ12_RS00660	105099..105971	+	873	Protein_128	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / estIa / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..225292	225292
	-	flank	IS/Tn	-	-	105099..105971	872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10780.46 Da
• Isoelectric Point: 9.5296

>T56522 WP_000421271.1 NZ_CP012500:c101040-100765 [Escherichia coli]
MELKWTSKALSDLERLYDFLALASKPAAARTVQSLTQAPIILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T56522 NZ_CP012500:c101040-100765 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGAGCGGTTATATGATTTTCTGGCGCTGGCCAGTAAACCAGC
TGCAGCCAGAACGGTGCAGTCCCTGACACAGGCACCGATCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGTGAGTACGAAATCCGTTACGAACTTACTGGTCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10550.99 Da
• Isoelectric Point: 5.6706

>AT56522 WP_001355337.1 NZ_CP012500:c101324-101040 [Escherichia coli]
MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWIIKQALSAWIAQEEERNRLTLEALDDVTSGRVIDHQAVQAW
ADSLDTDNSLPVPR

Nucleotide

• Length: 285 bp

>AT56522 NZ_CP012500:c101324-101040 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGGTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGATTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCACTGGACGATGTGACATCCGGACGGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGATACTGACAATTCGTTACCGGTGCCTCGCTGA

Structures

Toxin

• AlphaFold DB: A0A193MI35

Antitoxin

• AlphaFold DB: A0A0B1JM87