Detailed information of TA system TA056517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 47926..48551
• Replicon: plasmid pCFSAN004180G
• Accession: NZ_CP012500
• Organism: Escherichia coli strain 09-00049

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GJ12_RS00285
• Protein ID: WP_000911316.1
• Coordinates: 48153..48551 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: GJ12_RS00280
• Protein ID: WP_000450526.1
• Coordinates: 47926..48153 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ12_RS00280	47926..48153	+	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GJ12_RS00285	48153..48551	+	399	WP_000911316.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GJ12_RS00290	48560..50704	-	2145	WP_000009375.1	type IV conjugative transfer system coupling protein TraD	-
GJ12_RS30160	50760..51296	-	537	WP_001355323.1	hypothetical protein	-
GJ12_RS00300	51725..52204	-	480	WP_001355324.1	hypothetical protein	-
GJ12_RS00305	52408..53139	-	732	WP_001364068.1	complement resistance protein TraT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / estIa / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..225292	225292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14915.18 Da
• Isoelectric Point: 7.8605

>T56517 WP_000911316.1 NZ_CP012500:48153-48551 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T56517 NZ_CP012500:48153-48551 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACAATAAAGAACAAGCCCGCCAGTGTCAGAGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGACCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGCGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT56517 WP_000450526.1 NZ_CP012500:47926-48153 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT56517 NZ_CP012500:47926-48153 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGGCTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA