Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 2129..2651 Replicon plasmid pCFSAN004180G
Accession NZ_CP012500
Organism Escherichia coli strain 09-00049

Toxin (Protein)


Gene name relE Uniprot ID A0A0B0VZI6
Locus tag GJ12_RS00020 Protein ID WP_000220565.1
Coordinates 2129..2410 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0B0W3C1
Locus tag GJ12_RS00025 Protein ID WP_000121742.1
Coordinates 2400..2651 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ12_RS00015 583..1646 - 1064 Protein_1 IS256 family transposase -
GJ12_RS00020 2129..2410 - 282 WP_000220565.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GJ12_RS00025 2400..2651 - 252 WP_000121742.1 hypothetical protein Antitoxin
GJ12_RS00030 2926..3945 - 1020 WP_024166190.1 IS110 family transposase -
GJ12_RS30130 5322..5323 - 2 WP_085948398.1 IS3 family transposase -
GJ12_RS00050 5655..6456 - 802 Protein_6 IS256 family transposase -
GJ12_RS00055 6628..7248 - 621 WP_000269721.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / estIa / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC 1..225292 225292
- inside IScluster/Tn - - 5..22926 22921


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-82)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11037.83 Da        Isoelectric Point: 10.5938

>T56511 WP_000220565.1 NZ_CP012500:c2410-2129 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLSERLENPRVPAARLHGHADRYKIKLRSSGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T56511 NZ_CP012500:c2410-2129 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGTCAGAACGGCTGGAAAATCCACGTGTACCTGCAGCCCGATTACATGGTCATGCTGATCGTTATAAAATCA
AACTTCGTTCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGAGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9181.47 Da        Isoelectric Point: 4.2958

>AT56511 WP_000121742.1 NZ_CP012500:c2651-2400 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKKPEIVKVSL
DDL

Download         Length: 252 bp

>AT56511 NZ_CP012500:c2651-2400 [Escherichia coli]
ATGAGTTATCAAATCCTGACAACCACAGCGGCCAGTATTACTGACCTAAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GAGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAAACCGGAAATTGTGAAGGTTAGCCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B0VZI6

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0W3C1

References