Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
Location | 199138..199697 | Replicon | plasmid pCFSAN004181P |
Accession | NZ_CP012499 | ||
Organism | Escherichia coli strain GB089 |
Toxin (Protein)
Gene name | relE | Uniprot ID | A0A193MI35 |
Locus tag | CO57_RS01105 | Protein ID | WP_000421271.1 |
Coordinates | 199138..199413 (-) | Length | 92 a.a. |
Antitoxin (Protein)
Gene name | relB | Uniprot ID | A0A0B1JM87 |
Locus tag | CO57_RS01110 | Protein ID | WP_001355337.1 |
Coordinates | 199413..199697 (-) | Length | 95 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
CO57_RS01065 | 194786..195213 | - | 428 | Protein_211 | DUF1380 family protein | - |
CO57_RS01070 | 195209..195430 | - | 222 | WP_001104895.1 | hypothetical protein | - |
CO57_RS01075 | 195431..196114 | - | 684 | WP_000085877.1 | DNA methylase | - |
CO57_RS29845 | 196193..196494 | - | 302 | Protein_214 | hypothetical protein | - |
CO57_RS01085 | 196632..197594 | + | 963 | WP_000959884.1 | plasmid segregation protein ParM | - |
CO57_RS01090 | 197597..197947 | + | 351 | WP_024166183.1 | plasmid partitioning/stability family protein | - |
CO57_RS01095 | 198054..198515 | + | 462 | Protein_217 | hypothetical protein | - |
CO57_RS01100 | 198751..199032 | - | 282 | WP_001105063.1 | hypothetical protein | - |
CO57_RS01105 | 199138..199413 | - | 276 | WP_000421271.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
CO57_RS01110 | 199413..199697 | - | 285 | WP_001355337.1 | ribbon-helix-helix protein, CopG family | Antitoxin |
CO57_RS01115 | 200090..200311 | - | 222 | WP_001261037.1 | MarR family transcriptional regulator | - |
CO57_RS28095 | 200418..200823 | + | 406 | Protein_222 | hypothetical protein | - |
CO57_RS31325 | 200812..201081 | + | 270 | Protein_223 | integrase core domain-containing protein | - |
CO57_RS01125 | 201197..201787 | + | 591 | WP_001355339.1 | hypothetical protein | - |
CO57_RS01130 | 203472..204344 | + | 873 | Protein_225 | IS3 family transposase | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Conjugative plasmid | - | faeJ / faeI / faeH / faeF / faeE / faeD / faeC / hlyD / hlyB / hlyA / hlyC | 1..223952 | 223952 | |
- | flank | IS/Tn | - | - | 203472..204344 | 872 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10780.46 Da Isoelectric Point: 9.5296
>T56510 WP_000421271.1 NZ_CP012499:c199413-199138 [Escherichia coli]
MELKWTSKALSDLERLYDFLALASKPAAARTVQSLTQAPIILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLERLYDFLALASKPAAARTVQSLTQAPIILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T56510 NZ_CP012499:c199413-199138 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGAGCGGTTATATGATTTTCTGGCGCTGGCCAGTAAACCAGC
TGCAGCCAGAACGGTGCAGTCCCTGACACAGGCACCGATCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGTGAGTACGAAATCCGTTACGAACTTACTGGTCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGAGCGGTTATATGATTTTCTGGCGCTGGCCAGTAAACCAGC
TGCAGCCAGAACGGTGCAGTCCCTGACACAGGCACCGATCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGTGAGTACGAAATCCGTTACGAACTTACTGGTCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10550.99 Da Isoelectric Point: 5.6706
>AT56510 WP_001355337.1 NZ_CP012499:c199697-199413 [Escherichia coli]
MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWIIKQALSAWIAQEEERNRLTLEALDDVTSGRVIDHQAVQAW
ADSLDTDNSLPVPR
MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWIIKQALSAWIAQEEERNRLTLEALDDVTSGRVIDHQAVQAW
ADSLDTDNSLPVPR
Download Length: 285 bp
>AT56510 NZ_CP012499:c199697-199413 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGGTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGATTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCACTGGACGATGTGACATCCGGACGGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGATACTGACAATTCGTTACCGGTGCCTCGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGGTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGATTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCACTGGACGATGTGACATCCGGACGGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGATACTGACAATTCGTTACCGGTGCCTCGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A193MI35 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0B1JM87 |