Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 199138..199697 Replicon plasmid pCFSAN004181P
Accession NZ_CP012499
Organism Escherichia coli strain GB089

Toxin (Protein)


Gene name relE Uniprot ID A0A193MI35
Locus tag CO57_RS01105 Protein ID WP_000421271.1
Coordinates 199138..199413 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0B1JM87
Locus tag CO57_RS01110 Protein ID WP_001355337.1
Coordinates 199413..199697 (-) Length 95 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO57_RS01065 194786..195213 - 428 Protein_211 DUF1380 family protein -
CO57_RS01070 195209..195430 - 222 WP_001104895.1 hypothetical protein -
CO57_RS01075 195431..196114 - 684 WP_000085877.1 DNA methylase -
CO57_RS29845 196193..196494 - 302 Protein_214 hypothetical protein -
CO57_RS01085 196632..197594 + 963 WP_000959884.1 plasmid segregation protein ParM -
CO57_RS01090 197597..197947 + 351 WP_024166183.1 plasmid partitioning/stability family protein -
CO57_RS01095 198054..198515 + 462 Protein_217 hypothetical protein -
CO57_RS01100 198751..199032 - 282 WP_001105063.1 hypothetical protein -
CO57_RS01105 199138..199413 - 276 WP_000421271.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CO57_RS01110 199413..199697 - 285 WP_001355337.1 ribbon-helix-helix protein, CopG family Antitoxin
CO57_RS01115 200090..200311 - 222 WP_001261037.1 MarR family transcriptional regulator -
CO57_RS28095 200418..200823 + 406 Protein_222 hypothetical protein -
CO57_RS31325 200812..201081 + 270 Protein_223 integrase core domain-containing protein -
CO57_RS01125 201197..201787 + 591 WP_001355339.1 hypothetical protein -
CO57_RS01130 203472..204344 + 873 Protein_225 IS3 family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - faeJ / faeI / faeH / faeF / faeE / faeD / faeC / hlyD / hlyB / hlyA / hlyC 1..223952 223952
- flank IS/Tn - - 203472..204344 872


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 92 a.a.        Molecular weight: 10780.46 Da        Isoelectric Point: 9.5296

>T56510 WP_000421271.1 NZ_CP012499:c199413-199138 [Escherichia coli]
MELKWTSKALSDLERLYDFLALASKPAAARTVQSLTQAPIILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T56510 NZ_CP012499:c199413-199138 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGAGCGGTTATATGATTTTCTGGCGCTGGCCAGTAAACCAGC
TGCAGCCAGAACGGTGCAGTCCCTGACACAGGCACCGATCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGTGAGTACGAAATCCGTTACGAACTTACTGGTCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10550.99 Da        Isoelectric Point: 5.6706

>AT56510 WP_001355337.1 NZ_CP012499:c199697-199413 [Escherichia coli]
MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWIIKQALSAWIAQEEERNRLTLEALDDVTSGRVIDHQAVQAW
ADSLDTDNSLPVPR

Download         Length: 285 bp

>AT56510 NZ_CP012499:c199697-199413 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGGTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGATTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCACTGGACGATGTGACATCCGGACGGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGATACTGACAATTCGTTACCGGTGCCTCGCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A193MI35


Antitoxin

Source ID Structure
AlphaFold DB A0A0B1JM87

References