Detailed information of TA system TA056505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 147650..148275
• Replicon: plasmid pCFSAN004181P
• Accession: NZ_CP012499
• Organism: Escherichia coli strain GB089

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CO57_RS00760
• Protein ID: WP_000911316.1
• Coordinates: 147877..148275 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: CO57_RS00755
• Protein ID: WP_000450526.1
• Coordinates: 147650..147877 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO57_RS00755	147650..147877	+	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CO57_RS00760	147877..148275	+	399	WP_000911316.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CO57_RS00765	148284..150428	-	2145	WP_000009375.1	type IV conjugative transfer system coupling protein TraD	-
CO57_RS29820	150484..151020	-	537	WP_001355323.1	hypothetical protein	-
CO57_RS00775	151449..151928	-	480	WP_001355324.1	hypothetical protein	-
CO57_RS00780	152132..152863	-	732	WP_001364068.1	complement resistance protein TraT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeJ / faeI / faeH / faeF / faeE / faeD / faeC / hlyD / hlyB / hlyA / hlyC	1..223952	223952

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14915.18 Da
• Isoelectric Point: 7.8605

>T56505 WP_000911316.1 NZ_CP012499:147877-148275 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T56505 NZ_CP012499:147877-148275 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACAATAAAGAACAAGCCCGCCAGTGTCAGAGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGACCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGCGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT56505 WP_000450526.1 NZ_CP012499:147650-147877 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT56505 NZ_CP012499:147650-147877 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGGCTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA