Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 101852..102374 Replicon plasmid pCFSAN004181P
Accession NZ_CP012499
Organism Escherichia coli strain GB089

Toxin (Protein)


Gene name relE Uniprot ID A0A0B0VZI6
Locus tag CO57_RS00505 Protein ID WP_000220565.1
Coordinates 101852..102133 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0B0W3C1
Locus tag CO57_RS00510 Protein ID WP_000121742.1
Coordinates 102123..102374 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO57_RS00485 97740..97961 - 222 WP_032287374.1 hypothetical protein -
CO57_RS30580 99238..99393 - 156 Protein_97 ISKra4 family transposase -
CO57_RS00495 99453..100306 + 854 Protein_98 IS3 family transposase -
CO57_RS00500 100306..101369 - 1064 Protein_99 IS256 family transposase -
CO57_RS00505 101852..102133 - 282 WP_000220565.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CO57_RS00510 102123..102374 - 252 WP_000121742.1 hypothetical protein Antitoxin
CO57_RS00515 102649..103668 - 1020 WP_024166190.1 IS110 family transposase -
CO57_RS29790 105045..105046 - 2 WP_085948398.1 IS3 family transposase -
CO57_RS00535 105378..106179 - 802 Protein_104 IS256 family transposase -
CO57_RS00540 106351..106971 - 621 WP_000269721.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - faeJ / faeI / faeH / faeF / faeE / faeD / faeC / hlyD / hlyB / hlyA / hlyC 1..223952 223952
- inside IScluster/Tn - - 99453..122650 23197


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-82)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11037.83 Da        Isoelectric Point: 10.5938

>T56499 WP_000220565.1 NZ_CP012499:c102133-101852 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLSERLENPRVPAARLHGHADRYKIKLRSSGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T56499 NZ_CP012499:c102133-101852 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGTCAGAACGGCTGGAAAATCCACGTGTACCTGCAGCCCGATTACATGGTCATGCTGATCGTTATAAAATCA
AACTTCGTTCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGAGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9181.47 Da        Isoelectric Point: 4.2958

>AT56499 WP_000121742.1 NZ_CP012499:c102374-102123 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKKPEIVKVSL
DDL

Download         Length: 252 bp

>AT56499 NZ_CP012499:c102374-102123 [Escherichia coli]
ATGAGTTATCAAATCCTGACAACCACAGCGGCCAGTATTACTGACCTAAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GAGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAAACCGGAAATTGTGAAGGTTAGCCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B0VZI6

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0W3C1

References