Detailed information of TA system TA056494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 128417..129042
• Replicon: plasmid pCFSAN004178P_02
• Accession: NZ_CP012498
• Organism: Escherichia coli strain 06-00048 isolate CFSAN004178

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A193M1T7
• Locus tag: GJ11_RS01500
• Protein ID: WP_065225985.1
• Coordinates: 128417..128815 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: GJ11_RS01505
• Protein ID: WP_000450526.1
• Coordinates: 128815..129042 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ11_RS01480	123802..124533	+	732	WP_047670842.1	complement resistance protein TraT	-
GJ11_RS01485	124737..125216	+	480	WP_001355324.1	hypothetical protein	-
GJ11_RS01490	125645..126199	+	555	WP_123007686.1	hypothetical protein	-
GJ11_RS01495	126255..128408	+	2154	WP_065225984.1	type IV conjugative transfer system coupling protein TraD	-
GJ11_RS01500	128417..128815	-	399	WP_065225985.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GJ11_RS01505	128815..129042	-	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa / hlyC / hlyA / hlyB / hlyD / estIa / draB / afaC-I	1..213847	213847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15014.32 Da
• Isoelectric Point: 8.5264

>T56494 WP_065225985.1 NZ_CP012498:c128815-128417 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVRPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T56494 NZ_CP012498:c128815-128417 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACAATAAAGAACAAGCCCGCCAGTGTCAGAGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCAGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGCGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT56494 WP_000450526.1 NZ_CP012498:c129042-128815 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT56494 NZ_CP012498:c129042-128815 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGGCTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A193M1T7