Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 55504..56029 Replicon plasmid pCFSAN004178P_02
Accession NZ_CP012498
Organism Escherichia coli strain 06-00048 isolate CFSAN004178

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag GJ11_RS01080 Protein ID WP_001159871.1
Coordinates 55724..56029 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A193M1T0
Locus tag GJ11_RS01075 Protein ID WP_065225949.1
Coordinates 55504..55722 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ11_RS01060 51381..52820 + 1440 WP_065225947.1 enterohemolysin T1SS ABC transporter subunit EhxD -
GJ11_RS01065 52943..53464 + 522 Protein_53 MarR family transcriptional regulator -
GJ11_RS01075 55504..55722 + 219 WP_065225949.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GJ11_RS01080 55724..56029 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
GJ11_RS01085 56030..56835 + 806 Protein_57 phage integrase family protein -
GJ11_RS01090 57015..57659 + 645 WP_001144034.1 ParA family protein -
GJ11_RS01095 57746..58054 + 309 WP_065225950.1 molecular chaperone GroEL -
GJ11_RS01100 58468..59448 + 981 WP_000688515.1 plasmid segregation protein ParM -
GJ11_RS01105 59441..59857 + 417 WP_001278817.1 plasmid partitioning/stability family protein -
GJ11_RS01110 59859..60805 - 947 Protein_62 translesion error-prone DNA polymerase V subunit UmuC -
GJ11_RS01115 60839..60964 + 126 Protein_63 transposase zinc-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa / hlyC / hlyA / hlyB / hlyD / estIa / draB / afaC-I 1..213847 213847


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T56492 WP_001159871.1 NZ_CP012498:55724-56029 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T56492 NZ_CP012498:55724-56029 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTAGCCAGCGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTCATTGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8314.31 Da        Isoelectric Point: 5.0506

>AT56492 WP_065225949.1 NZ_CP012498:55504-55722 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYGVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT56492 NZ_CP012498:55504-55722 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACAGTCGACAGTGACAGCTATCAGTTGCTCAAAGCATATGGTGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A193M1T0

References