Detailed information of TA system TA056491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 37215..37762
• Replicon: plasmid pCFSAN004178P_02
• Accession: NZ_CP012498
• Organism: Escherichia coli strain 06-00048 isolate CFSAN004178

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: GJ11_RS00985
• Protein ID: WP_065225937.1
• Coordinates: 37481..37762 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A193M1P9
• Locus tag: GJ11_RS00980
• Protein ID: WP_065225936.1
• Coordinates: 37215..37484 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ11_RS00955	32327..33028	+	702	WP_065225934.1	fumarylacetoacetate hydrolase family protein	-
GJ11_RS00960	33037..33681	+	645	WP_062904986.1	maleylacetoacetate isomerase	-
GJ11_RS00965	33696..34889	+	1194	WP_065225935.1	3-hydroxybenzoate 6-monooxygenase	-
GJ11_RS00970	35117..36474	+	1358	WP_085950990.1	IS3-like element ISEcB1 family transposase	-
GJ11_RS00975	36584..36772	+	189	WP_024221770.1	hypothetical protein	-
GJ11_RS00980	37215..37484	+	270	WP_065225936.1	hypothetical protein	Antitoxin
GJ11_RS00985	37481..37762	+	282	WP_065225937.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJ11_RS00990	37917..38255	+	339	WP_185762086.1	hypothetical protein	-
GJ11_RS00995	40223..40364	-	142	Protein_39	IS3 family transposase	-
GJ11_RS01005	41733..42018	-	286	Protein_41	hypothetical protein	-
GJ11_RS01010	42125..42346	+	222	WP_001261037.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa / hlyC / hlyA / hlyB / hlyD / estIa / draB / afaC-I	1..213847	213847
	-	inside	IScluster/Tn	-	-	35117..41551	6434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10903.52 Da
• Isoelectric Point: 7.3985

>T56491 WP_065225937.1 NZ_CP012498:37481-37762 [Escherichia coli]
MMDIFWTMLASQDRKHIREYIAEQNLMSAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T56491 NZ_CP012498:37481-37762 [Escherichia coli]
ATGATGGATATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCACATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GTCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAATCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTATTGCATACCGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10239.58 Da
• Isoelectric Point: 6.4681

>AT56491 WP_065225936.1 NZ_CP012498:37215-37484 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLELGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT56491 NZ_CP012498:37215-37484 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGATCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGTCGTCAGCAACTCGAACTCGGTGATGTGCTTCCCCATGATATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A193M1P9