Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 57048..57649 Replicon plasmid pCFSAN004178P_01
Accession NZ_CP012497
Organism Escherichia coli strain 06-00048 isolate CFSAN004178

Toxin (Protein)


Gene name doc Uniprot ID A0A193LS81
Locus tag GJ11_RS00280 Protein ID WP_052957703.1
Coordinates 57048..57428 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag GJ11_RS00285 Protein ID WP_001190712.1
Coordinates 57428..57649 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ11_RS29050 52175..52405 - 231 Protein_49 ash family protein -
GJ11_RS00255 (GJ11_p00255) 52489..53973 - 1485 WP_000124159.1 hypothetical protein -
GJ11_RS00260 (GJ11_p00260) 53973..55166 - 1194 WP_065226842.1 terminase -
GJ11_RS00265 (GJ11_p00265) 55252..55626 - 375 WP_001339208.1 hypothetical protein -
GJ11_RS00270 (GJ11_p00270) 55793..56836 - 1044 WP_065226841.1 DUF968 domain-containing protein -
GJ11_RS00275 (GJ11_p00275) 56864..57043 - 180 WP_001697572.1 hypothetical protein -
GJ11_RS00280 (GJ11_p00280) 57048..57428 - 381 WP_052957703.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
GJ11_RS00285 (GJ11_p00285) 57428..57649 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GJ11_RS00290 (GJ11_p00290) 57722..58111 - 390 WP_046660029.1 S24 family peptidase -
GJ11_RS00295 58913..59578 - 666 WP_097463134.1 hypothetical protein -
GJ11_RS00300 (GJ11_p00300) 59575..59781 - 207 WP_065226840.1 hypothetical protein -
GJ11_RS00305 59774..59932 - 159 Protein_60 DUF551 domain-containing protein -
GJ11_RS00310 (GJ11_p00310) 60050..60778 - 729 WP_065226839.1 hypothetical protein -
GJ11_RS00315 (GJ11_p00320) 60973..61479 - 507 WP_065226838.1 3'-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..136480 136480


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13558.31 Da        Isoelectric Point: 5.6343

>T56490 WP_052957703.1 NZ_CP012497:c57428-57048 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSTVAATLRRLYGSAE

Download         Length: 381 bp

>T56490 NZ_CP012497:c57428-57048 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCAGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTACTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT56490 WP_001190712.1 NZ_CP012497:c57649-57428 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT56490 NZ_CP012497:c57649-57428 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACTCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CCGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTAAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193LS81

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References