Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 20221..20746 Replicon plasmid pCFSAN004176P_01
Accession NZ_CP012492
Organism Escherichia coli strain CFSAN004176

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag BCV59_RS00725 Protein ID WP_001159871.1
Coordinates 20441..20746 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag BCV59_RS00720 Protein ID WP_000813634.1
Coordinates 20221..20439 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCV59_RS30795 16750..16842 - 93 Protein_20 IS66 family insertion sequence element accessory protein TnpB -
BCV59_RS00685 16839..17264 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
BCV59_RS00690 17346..17684 - 339 WP_185768398.1 hypothetical protein -
BCV59_RS27790 17752..17928 + 177 Protein_23 hypothetical protein -
BCV59_RS00695 17950..18285 - 336 WP_069356957.1 colicin transporter -
BCV59_RS00700 18414..18761 + 348 WP_069356958.1 hypothetical protein -
BCV59_RS00705 18779..19369 - 591 WP_069356959.1 hypothetical protein -
BCV59_RS00710 19369..19626 - 258 WP_000343099.1 hypothetical protein -
BCV59_RS30800 19723..19977 + 255 WP_158707834.1 hypothetical protein -
BCV59_RS00720 20221..20439 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BCV59_RS00725 20441..20746 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
BCV59_RS00730 20747..21553 + 807 WP_069356961.1 site-specific integrase -
BCV59_RS00735 21915..23138 - 1224 WP_172907135.1 RepB family plasmid replication initiator protein -
BCV59_RS00745 23323..24535 + 1213 Protein_33 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - papC / papD 1..34714 34714


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T56489 WP_001159871.1 NZ_CP012492:20441-20746 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T56489 NZ_CP012492:20441-20746 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGGGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT56489 WP_000813634.1 NZ_CP012492:20221-20439 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT56489 NZ_CP012492:20221-20439 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCTCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References