Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 233..1008 Replicon plasmid pCFSAN004176P_01
Accession NZ_CP012492
Organism Escherichia coli strain CFSAN004176

Toxin (Protein)


Gene name tacT Uniprot ID A0A8D9PPA8
Locus tag BCV59_RS00585 Protein ID WP_000405244.1
Coordinates 526..1008 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag BCV59_RS00580 Protein ID WP_001346256.1
Coordinates 233..535 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCV59_RS00580 233..535 + 303 WP_001346256.1 DUF1778 domain-containing protein Antitoxin
BCV59_RS00585 526..1008 + 483 WP_000405244.1 GNAT family N-acetyltransferase Toxin
BCV59_RS00590 1318..1575 + 258 WP_000083826.1 replication regulatory protein RepA -
BCV59_RS00595 1808..1882 + 75 WP_001365705.1 RepA leader peptide Tap -
BCV59_RS00600 1875..2732 + 858 WP_069356946.1 incFII family plasmid replication initiator RepA -
BCV59_RS00610 3632..3901 + 270 WP_032191903.1 hypothetical protein -
BCV59_RS00620 4097..5305 - 1209 WP_069356948.1 IS91 family transposase -
BCV59_RS27770 5286..5558 - 273 WP_071961460.1 hypothetical protein -
BCV59_RS27775 5638..5874 - 237 Protein_8 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - papC / papD 1..34714 34714


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-149)

Antitoxin

Vibrio phage ICP1, Orf50

(15-99)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17659.41 Da        Isoelectric Point: 7.1036

>T56488 WP_000405244.1 NZ_CP012492:526-1008 [Escherichia coli]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLHGRAMKNQMLNASRTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T56488 NZ_CP012492:526-1008 [Escherichia coli]
ATGGAAATAAATGTCACCGCACCGGCATTACTGACGGATGAGCATATACTTCAGCCATTTGATTGTGGGAATGAAGTGTT
AAGTAACTGGTTACATGGTCGCGCTATGAAAAACCAGATGCTCAATGCTTCCCGCACGTTCGTCATTTGCCTGGAGGGTA
CTTTACGTGTTGTGGGGTACTACTCATTAGCTACCGGTTCCGTCACTCATGCCGAACTTGGGCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTTCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACTCAGGGGCATGGTTTTGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGGTTGTCAATCTGGCTGATCAGGTTGGTATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGATGCCAGAGCGTTTTATGAGCGTTTTGGTTTTGTCCAGTCAGTTGTGGCACCAAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11602.19 Da        Isoelectric Point: 4.4569

>AT56488 WP_001346256.1 NZ_CP012492:233-535 [Escherichia coli]
MEVVMPTTASIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT56488 NZ_CP012492:233-535 [Escherichia coli]
ATGGAGGTTGTTATGCCTACTACTGCAAGCATCCGTAAAACACCGCGAGAAAATCAGATTAACATCCGGGCAACGGATGA
GGAACGAGCCGTTATTGATTATGCCGCTAGCCTGGTCAATAAGAACCGTACTGATTTCATCATTGAGCTGGCTTACCAGG
AAGCTAAAAACATCATCCTGGATCAACGTTTATTTGTGCTGGATGATGAATGTTATGATTCATTTATTACGCAACTTGAA
GCTCCTGTGCAGAATGCTGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References