Detailed information of TA system TA056487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 63907..64508
• Replicon: plasmid pCFSAN004176P_03
• Accession: NZ_CP012491
• Organism: Escherichia coli strain CFSAN004176

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: BCV59_RS00430
• Protein ID: WP_001216045.1
• Coordinates: 64128..64508 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: BCV59_RS00425
• Protein ID: WP_001190712.1
• Coordinates: 63907..64128 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCV59_RS00380	59002..59259	+	258	WP_000382636.1	hypothetical protein	-
BCV59_RS00385	59259..59540	+	282	WP_000002126.1	ASCH domain-containing protein	-
BCV59_RS00390	59564..59857	+	294	WP_000267998.1	hypothetical protein	-
BCV59_RS00395	59864..60238	+	375	WP_000988652.1	hypothetical protein	-
BCV59_RS00400	60220..60894	+	675	WP_000057460.1	hypothetical protein	-
BCV59_RS00405	60891..61253	+	363	WP_001261541.1	hypothetical protein	-
BCV59_RS00410	62329..62685	-	357	WP_001062547.1	hypothetical protein	-
BCV59_RS00415	62686..63270	-	585	WP_001133673.1	hypothetical protein	-
BCV59_RS00420	63445..63834	+	390	WP_000506724.1	DNA repair protein	-
BCV59_RS00425	63907..64128	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BCV59_RS00430	64128..64508	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
BCV59_RS00435	64513..64692	+	180	WP_000113018.1	hypothetical protein	-
BCV59_RS00440	64720..65763	+	1044	WP_000648833.1	DUF968 domain-containing protein	-
BCV59_RS00445	65852..66304	+	453	WP_001326849.1	late promoter-activating protein	-
BCV59_RS00450	66390..67583	+	1194	WP_000219618.1	hypothetical protein	-
BCV59_RS00455	67583..69067	+	1485	WP_000124159.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95721	95721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T56487 WP_001216045.1 NZ_CP012491:64128-64508 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T56487 NZ_CP012491:64128-64508 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT56487 WP_001190712.1 NZ_CP012491:63907-64128 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT56487 NZ_CP012491:63907-64128 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6