Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5188622..5189264 Replicon chromosome
Accession NZ_CP012483
Organism Bacillus cereus strain NJ-W

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag ACN91_RS26440 Protein ID WP_000635965.1
Coordinates 5188622..5188972 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag ACN91_RS26445 Protein ID WP_000004570.1
Coordinates 5188977..5189264 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACN91_RS26425 5185557..5186015 - 459 WP_000503730.1 SprT family protein -
ACN91_RS26430 5186211..5186327 + 117 WP_001143640.1 cortex morphogenetic protein CmpA -
ACN91_RS26435 5186386..5188554 - 2169 WP_000426207.1 RNA-binding transcriptional accessory protein -
ACN91_RS26440 5188622..5188972 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ACN91_RS26445 5188977..5189264 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
ACN91_RS26450 5189573..5190742 - 1170 WP_000390593.1 alanine racemase -
ACN91_RS26455 5190860..5191810 - 951 WP_002094231.1 outer membrane lipoprotein carrier protein LolA -
ACN91_RS26460 5191967..5192326 - 360 WP_053565408.1 holo-ACP synthase -
ACN91_RS26465 5192420..5192992 + 573 WP_000908526.1 rhomboid family intramembrane serine protease -
ACN91_RS26470 5192985..5193947 - 963 WP_000961144.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T56486 WP_000635965.1 NZ_CP012483:c5188972-5188622 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T56486 NZ_CP012483:c5188972-5188622 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT56486 WP_000004570.1 NZ_CP012483:c5189264-5188977 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT56486 NZ_CP012483:c5189264-5188977 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References