Detailed information of TA system TA056485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3140911..3141889
• Replicon: chromosome
• Accession: NZ_CP012483
• Organism: Bacillus cereus strain NJ-W

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: ACN91_RS15970
• Protein ID: WP_053564491.1
• Coordinates: 3141152..3141889 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: ACN91_RS15965
• Protein ID: WP_000237818.1
• Coordinates: 3140911..3141039 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACN91_RS15940	3136891..3137493	-	603	WP_053564489.1	hypothetical protein	-
ACN91_RS15945	3137519..3138328	-	810	WP_053564490.1	C39 family peptidase	-
ACN91_RS15950	3138574..3140043	-	1470	WP_048656315.1	beta-Ala-His dipeptidase	-
ACN91_RS15955	3140255..3140644	+	390	WP_000713869.1	YxeA family protein	-
ACN91_RS15960	3140662..3140838	-	177	WP_000852629.1	stage II sporulation protein SB	-
ACN91_RS15965	3140911..3141039	-	129	WP_000237818.1	hypothetical protein	Antitoxin
ACN91_RS15970	3141152..3141889	-	738	WP_053564491.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
ACN91_RS15975	3142128..3142865	-	738	WP_053564492.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
ACN91_RS15980	3143032..3143514	-	483	WP_053564493.1	MarR family winged helix-turn-helix transcriptional regulator	-
ACN91_RS15985	3143631..3145307	+	1677	WP_053564494.1	alpha-keto acid decarboxylase family protein	-
ACN91_RS15990	3145465..3146247	-	783	WP_053564495.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.3184

>T56485 WP_053564491.1 NZ_CP012483:c3141889-3141152 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFFWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPSVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T56485 NZ_CP012483:c3141889-3141152 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTTTTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACTGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCAGAAATATGGGGTGCGAAATTTAGTGATATTGGCAAGACAGTTCAATCGATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCATCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT56485 WP_000237818.1 NZ_CP012483:c3141039-3140911 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT56485 NZ_CP012483:c3141039-3140911 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5