56482

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 119..726 Replicon plasmid ISMMS_VRE7_p3
Accession NZ_CP012463
Organism Enterococcus faecium strain ISMMS_VRE_7

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag AL020_RS15455 Protein ID WP_002321032.1
Coordinates 119..469 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag AL020_RS15460 Protein ID WP_002287514.1
Coordinates 463..726 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL020_RS15455 119..469 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AL020_RS15460 463..726 - 264 WP_002287514.1 hypothetical protein Antitoxin
AL020_RS15465 964..2109 - 1146 WP_002305757.1 IS200/IS605 family element transposase accessory protein TnpB -
AL020_RS15470 2121..2522 - 402 WP_002305759.1 IS200/IS605 family transposase -
AL020_RS15475 2689..2961 - 273 WP_002348912.1 hypothetical protein -
AL020_RS15480 2943..3359 - 417 WP_002305764.1 hypothetical protein -
AL020_RS15485 3375..3626 - 252 WP_002305766.1 hypothetical protein -
AL020_RS15490 3626..3832 - 207 WP_002296239.1 hypothetical protein -
AL020_RS15495 4277..4537 - 261 WP_002321303.1 hypothetical protein -
AL020_RS15500 4903..5376 - 474 WP_002321302.1 single-stranded DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..54805 54805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T56482 WP_002321032.1 NZ_CP012463:c469-119 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T56482 NZ_CP012463:c469-119 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT56482 WP_002287514.1 NZ_CP012463:c726-463 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT56482 NZ_CP012463:c726-463 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References