Detailed information of TA system TA056479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 1795304..1795875
• Replicon: chromosome
• Accession: NZ_CP012460
• Organism: Enterococcus faecium strain ISMMS_VRE_7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3Y2B8
• Locus tag: AL020_RS08960
• Protein ID: WP_002286801.1
• Coordinates: 1795304..1795645 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AL020_RS08965
• Protein ID: WP_033659738.1
• Coordinates: 1795645..1795875 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL020_RS08940	1790581..1791411	+	831	WP_002286789.1	manganese catalase family protein	-
AL020_RS16455	1791655..1791937	-	283	Protein_1730	transposase	-
AL020_RS08950	1792095..1792289	-	195	WP_002297028.1	hypothetical protein	-
AL020_RS08955	1793083..1794270	+	1188	WP_002296840.1	IS256-like element IS16 family transposase	-
AL020_RS08960	1795304..1795645	-	342	WP_002286801.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AL020_RS08965	1795645..1795875	-	231	WP_033659738.1	hypothetical protein	Antitoxin
AL020_RS08970	1796162..1796500	+	339	WP_002286804.1	hypothetical protein	-
AL020_RS08975	1796705..1796944	-	240	WP_002321938.1	SOS response-associated peptidase family protein	-
AL020_RS08980	1796920..1797267	-	348	WP_002286808.1	SOS response-associated peptidase family protein	-
AL020_RS08985	1797999..1798580	-	582	WP_002286813.1	TetR/AcrR family transcriptional regulator	-
AL020_RS08990	1798763..1799389	-	627	WP_002286816.1	cysteine hydrolase	-
AL020_RS08995	1799411..1800742	-	1332	WP_002286818.1	FAD-containing oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1793083..1794270	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13302.68 Da
• Isoelectric Point: 9.9044

>T56479 WP_002286801.1 NZ_CP012460:c1795645-1795304 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T56479 NZ_CP012460:c1795645-1795304 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8451.32 Da
• Isoelectric Point: 3.9490

>AT56479 WP_033659738.1 NZ_CP012460:c1795875-1795645 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIGDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT56479 NZ_CP012460:c1795875-1795645 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGGAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A829FD66